Taxonomic and Enzymatic Characterization of Flocculibacter collagenilyticus gen. nov., sp. nov., a Novel Gammaproteobacterium With High Collagenase Production.
Taxonomic and Enzymatic Characterization of Flocculibacter collagenilyticus gen. nov., sp. nov., a Novel Gammaproteobacterium With High Collagenase Production.
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溶胶原絮状杆菌属的分类学和酶学特征。
DOI：
10.3389/fmicb.2021.621161
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发表时间：
2021
影响因子：
5.2
通讯作者：
Zhang XY
中科院分区：
文献类型：
--
作者：
Li J;Cheng JH;Teng ZJ;Sun ZZ;He XY;Wang P;Shi M;Song XY;Chen XL;Zhang YZ;Tian X;Zhang XY
来自海洋动物的胶原蛋白是海洋有机氮的重要组成部分。产生胶原酶的细菌及其胶原酶在海洋胶原蛋白降解和有机氮回收中发挥着重要作用。然而，迄今为止只发现了少数产生胶原酶的海洋细菌。在这里，我们报道了从绿藻 Codium 脆弱样品中分离出的胶原酶分泌细菌（指定菌株 SM1988T）的分离和表征。 SM1988T 菌株是一种革兰氏阴性、好氧、氧化酶和过氧化氢酶阳性、单极有鞭毛和杆状细菌，能够水解酪蛋白、明胶和胶原蛋白。系统发育分析表明，菌株SM1988T与假互单胞菌科内的已知属形成了独特的系统发育谱系，其16S rRNA基因序列与该科中所有已知物种的相似性低于93.3%。基于系统发育、基因组、化学分类学和表型数据，菌株SM1988T被认为代表了假交替单胞菌科新属中的新种，其名称为溶胶原絮状杆菌属。十一月，SP。十一月建议菌株为 SM1988T (= MCCC 1K04279T = KCTC 72761T)。菌株 SM1988T 显示出高产量的细胞外胶原酶，其对牛胶原蛋白和鳕鱼胶原蛋白均具有高活性。生化测试结合基因组和分泌组分析表明菌株SM1988T分泌的胶原酶是来自MEROPS S8家族的丝氨酸蛋白酶。这些数据表明菌株SM1988T在海洋胶原蛋白降解和回收中发挥着重要作用，并且在胶原蛋白资源利用方面可能具有广阔的潜力。
Collagens from marine animals are an important component of marine organic nitrogen. Collagenase-producing bacteria and their collagenases play important roles in collagen degradation and organic nitrogen recycling in the ocean. However, only a few collagenase-producing marine bacteria have been so far discovered. Here, we reported the isolation and characterization of a collagenase-secreting bacterium, designated strain SM1988T, isolated from a green alga Codium fragile sample. Strain SM1988T is a Gram-negative, aerobic, oxidase-, and catalase-positive, unipolar flagellated, and rod-shaped bacterium capable of hydrolyzing casein, gelatin and collagens. Phylogenetic analysis revealed that strain SM1988T formed a distinct phylogenetic lineage along with known genera within the family Pseudoalteromonadaceae, with 16S rRNA gene sequence similarity being less than 93.3% to all known species in the family. Based on the phylogenetic, genomic, chemotaxonomic and phenotypic data, strain SM1988T was considered to represent a novel species in a novel genus in the family Pseudoalteromonadaceae, for which the name Flocculibacter collagenilyticus gen. nov., sp. nov. is proposed, with the type strain being SM1988T (= MCCC 1K04279T = KCTC 72761T). Strain SM1988T showed a high production of extracellular collagenases, which had high activity against both bovine collagen and codfish collagen. Biochemical tests combined with genome and secretome analyses indicated that the collagenases secreted by strain SM1988T are serine proteases from the MEROPS S8 family. These data suggest that strain SM1988T acts as an important player in marine collagen degradation and recycling and may have a promising potential in collagen resource utilization.
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DOI：
10.1099/ijs.0.64963-0
发表时间：
2008-09-01
影响因子：
2.8
作者：
Al Khudary, Rami;Stoesser, Nele Isabelle;Antranikian, Garabed
通讯作者：
Antranikian, Garabed
影响因子：
2.8
作者：
Fujino, Masashi;Ishihara, Masaharu;Ogawa, Hisao
通讯作者：
Ogawa, Hisao
影响因子：
2.9
作者：
HEUSSEN, C;DOWDLE, EB
通讯作者：
DOWDLE, EB
模因套件：用于发现和搜索的工具。
影响因子：
14.9
作者：
Bailey TL;Boden M;Buske FA;Frith M;Grant CE;Clementi L;Ren J;Li WW;Noble WS
通讯作者：
Noble WS
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro