A 1H-NMR Based Study on Hemolymph Metabolomics in Eri Silkworm after Oral Administration of 1-Deoxynojirimycin.
A 1H-NMR Based Study on Hemolymph Metabolomics in Eri Silkworm after Oral Administration of 1-Deoxynojirimycin.
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基于1H-NMR的Eri蚕口服1-脱氧野尻霉素后血淋巴代谢组学研究
DOI：
10.1371/journal.pone.0131696
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Xu JP
中科院分区：
文献类型：
--
作者：
Deng MJ;Lin XD;Lin QT;Wen DF;Zhang ML;Wang XQ;Gao HC;Xu JP
我们的目的是研究 1-脱氧野尻霉素 (DNJ) 是否调节糖代谢并对 Eri 蚕（Samia cynthia ricini，Saturniidae）具有毒性。本文利用血淋巴代谢物探讨口服DNJ或桑乳胶后的代谢变化，并在代谢和全身水平上表征DNJ的生物学功能。从 Eri 蚕的四龄幼虫中收集血淋巴样本，并从收集的血淋巴样本中获取离体高分辨率 1H 核磁共振 (NMR) 谱。然后通过主成分分析（PCA）和独立样本t检验对获得的光谱进行分析。血淋巴样本的代谢模式识别分析表明，0.25％DNJ、乳胶以及0.5％DNJ和乳胶(1:1)混合物组与对照组有显着差异。此外，与对照组相比，0.25% DNJ、乳胶、0.5% DNJ 与乳胶混合物（1:1）组血淋巴中柠檬酸盐、琥珀酸盐、富马酸盐、苹果酸盐、谷氨酰胺水平降低，0.25% DNJ、0.5% DNJ 与乳胶混合物（1:1）组海藻糖和谷氨酰胺水平升高。乳酸盐。此外，桑叶分泌的乳胶对埃里蚕具有剧毒，因为桑叶中含有丰富的不明高分子因子，是有毒物质。在我们的结果中，乳胶导致 50 只埃里蚕蛾四龄幼虫中的 20 只死亡，但 DNJ 或混合物并未导致死亡。所有这些结果表明，DNJ 通过调节糖代谢、抑制糖生成和能量代谢，对逆糖代谢产生积极影响。由于其无毒和生物活性，DNJ 作为单成分抗糖尿病药物是一种安全的物质。
We aimed to investigate whether 1-deoxynojirimycin (DNJ) modulates glycometabolism and has toxicity in Eri silkworm (Samia cynthia ricini, Saturniidae). In this paper, hemolymph metabolites were used to explore metabolic changes after oral administration of DNJ or mulberry latex and to characterize the biological function of DNJ at the metabolic and systemic levels. Hemolymph samples were collected from fourth-instar larvae of Eri silkworm and ex-vivo high-resolution 1H nuclear magnetic resonance (NMR) spectra were acquired from the collected hemolymph samples. Then the obtained spectra were analyzed by principal component analysis (PCA) and independent-samples t-test. Metabolic pattern recognition analysis of hemolymph samples indicated that the groups of 0.25% DNJ, latex, and the mixture of 0.5% DNJ and latex (1:1) were significantly different from the control group. Moreover, compared to the control group, the groups of 0.25% DNJ, latex, and the mixture of 0.5% DNJ and latex (1:1) showed the decreased levels of citrate, succinate, fumarate, malate, and glutamine in hemolymph, the groups of 0.25% DNJ and the mixture of 0.5% DNJ and latex (1:1) showed the increased levels of trehalose and lactate. In addition, mulberry leaves exude latex was highly toxic to Eri silkworm because rich unidentified high-molecular-weight factor (s) acted as toxic substances. In our results, latex caused 20 deaths among 50 fourth-instar larvae of Eri silkmoth, but DNJ or the mixture did not caused death. All these results suggest that DNJ has a positive impact on the reverse glycometabolism by modulating glycometabolism and inhibiting glucogenesis and energy metabolism. DNJ is a secure substance as a single-ingredient antidiabetic medicine due to its nontoxicity and bioactivity.
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影响因子：
15.9
作者：
LEVINE, RJ;CONN, HO
通讯作者：
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影响因子：
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作者：
Li YG;Ji DF;Zhong S;Lv ZQ;Lin TB
通讯作者：
Lin TB
影响因子：
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作者：
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通讯作者：
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影响因子：
5.4
作者：
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通讯作者：
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影响因子：
2.2
作者：
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通讯作者：
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