Comparative proteomic analyses provide new insights into low phosphorus stress responses in maize leaves.
Comparative proteomic analyses provide new insights into low phosphorus stress responses in maize leaves.
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比较蛋白质组分析为玉米叶片低磷胁迫反应提供新见解
DOI：
10.1371/journal.pone.0098215
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Chen H
中科院分区：
文献类型：
--
作者：
Zhang K;Liu H;Tao P;Chen H
缺磷限制植物的生长发育。为了更好地了解玉米响应磷酸盐胁迫的机制，我们比较了分别用1，000 µM（对照，+P）和5 µM KH 2 PO 4（干预组，-P）处理25天的两组玉米叶片的蛋白质组分析结果。在2-DE图谱上共检测到1，342个蛋白质点，15.43%的蛋白质点在+P组和−P组之间发生了显著变化（P<0.05; ≥1.5倍）。这些蛋白质参与几种主要的代谢途径，包括光合作用、碳水化合物代谢、能量代谢、次生代谢、信号转导、蛋白质合成、细胞拯救和细胞防御以及毒力。结果表明，低磷胁迫下光合作用降低是由于参与CO2富集的蛋白质、卡尔文循环和电子传递系统表达下调所致。电子传递和光合作用的限制，导致大量积累的过氧化物。玉米在低磷胁迫下通过提高抗氧化物质含量和抗氧化酶活性等多种途径清除活性氧。玉米在长期磷胁迫下，可通过改变光呼吸、淀粉合成和脂类组成来提高内源磷的利用效率。这些结果为玉米对低磷胁迫的反应提供了重要信息。
Phosphorus deficiency limits plant growth and development. To better understand the mechanisms behind how maize responds to phosphate stress, we compared the proteome analysis results of two groups of maize leaves that were treated separately with 1,000 µM (control, +P) and 5 µM of KH2PO4 (intervention group, −P) for 25 days. In total, 1,342 protein spots were detected on 2-DE maps and 15.43% had changed (P<0.05; ≥1.5-fold) significantly in quantity between the +P and −P groups. These proteins are involved in several major metabolic pathways, including photosynthesis, carbohydrate metabolism, energy metabolism, secondary metabolism, signal transduction, protein synthesis, cell rescue and cell defense and virulence. The results showed that the reduction in photosynthesis under low phosphorus treatment was due to the down-regulation of the proteins involved in CO2 enrichment, the Calvin cycle and the electron transport system. Electron transport and photosynthesis restrictions resulted in a large accumulation of peroxides. Maize has developed many different reactive oxygen species (ROS) scavenging mechanisms to cope with low phosphorus stress, including up-regulating its antioxidant content and antioxidase activity. After being subjected to phosphorus stress over a long period, maize may increase its internal phosphorus utilization efficiency by altering photorespiration, starch synthesis and lipid composition. These results provide important information about how maize responds to low phosphorus stress.
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影响因子：
7.2
作者：
Igarashi, D;Miwa, T;Ohsumi, C
通讯作者：
Ohsumi, C
DOI：
10.3839/jksabc.2011.102
发表时间：
2011-10-01
影响因子：
--
作者：
Kim, Sang Gon;Wang, Yiming;Kim, Sun Tae
通讯作者：
Kim, Sun Tae
影响因子：
6.9
作者：
Hammond, John P.;White, Philip J.
通讯作者：
White, Philip J.
影响因子：
3.7
作者：
Hammond JP;Broadley MR;Bowen HC;Spracklen WP;Hayden RM;White PJ
通讯作者：
White PJ
影响因子：
6.9
作者：
Calderon-Vazquez, Carlos;Ibarra-Laclette, Enrique;Herrera-Estrella, Luis
通讯作者：
Herrera-Estrella, Luis