Inhibiting biofilm formation by Klebsiella pneumoniae B5055 using an iron antagonizing molecule and a bacteriophage.
Inhibiting biofilm formation by Klebsiella pneumoniae B5055 using an iron antagonizing molecule and a bacteriophage.
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DOI：
10.1186/1471-2180-13-174
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发表时间：
2013-07-26
期刊：
影响因子：
4.2
通讯作者：
Bansal S
中科院分区：
文献类型：
--
作者：
Chhibber S;Nag D;Bansal S
生物膜驻留细菌在引起持续性和慢性感染方面的成功归因于它们对抗生素和免疫防御的抗性。游离铁对生物膜相关细菌的生长至关重要。因此，在本研究中，通过添加二价Co[II]离子与噬菌体组合来限制铁水平的效果用于预防/破坏肺炎克雷伯氏菌生物膜。与单独使用任何一种试剂相比，观察到用Co[II]和解聚酶产生噬菌体组合处理的较年轻和较年长生物膜中细菌数量的显著更高减少（p < 0.005）。证实了噬菌体携带的解聚酶的作用，因为观察到非解聚酶产生噬菌体与钴离子组合对生物膜的不显著根除。通过目视检查生物膜进一步确认活菌计数结果。从研究可以得出结论，铁拮抗分子和噬菌体可以用作预防生物膜发展的辅助疗法。
Success of biofilm dwelling bacteria in causing persistent and chronic infections is attributed to their resistance towards antibiotics and immune defences. Free iron is critical for the growth of biofilm associated bacteria. Therefore in the present study, the effect of limiting iron levels by addition of divalent Co[II] ions in combination with a bacteriophage was used for preventing/disrupting Klebsiella pneumoniae biofilms. A significantly higher reduction (p < 0.005) in bacterial numbers in the younger as well as older biofilms treated with Co[II] and depolymerase producing phage in combination was observed in comparison to when either of the agents was used alone. The role of phage borne depolymerase was confirmed, as an insignificant eradication of biofilm by non-depolymerase producing bacteriophage in combination with cobalt ions was observed. The results of viable count were further confirmed by visual examination of biofilms. From the study it can be concluded, that iron antagonizing molecules and bacteriophages can be used as adjunct therapy for preventing biofilm development.
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影响因子：
--
作者：
Musk, DJ;Banko, DA;Hergenrother, PJ
通讯作者：
Hergenrother, PJ
影响因子：
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影响因子：
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作者：
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影响因子：
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作者：
Verma, Vivek;Harjai, Kusum;Chhibber, Sanjay
通讯作者：
Chhibber, Sanjay
DOI：
10.1177/039463200501800407
发表时间：
2005-10-01
影响因子：
3.5
作者：
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通讯作者：
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