Successful high-level accumulation of fish oil omega-3 long-chain polyunsaturated fatty acids in a transgenic oilseed crop.
Successful high-level accumulation of fish oil omega-3 long-chain polyunsaturated fatty acids in a transgenic oilseed crop.
复制标题
DOI：
10.1111/tpj.12378
复制
发表时间：
2014-01
期刊：
影响因子：
--
通讯作者：
Sayanova O
中科院分区：
文献类型：
--
作者：
Ruiz-Lopez N;Haslam RP;Napier JA;Sayanova O
Omega-3(也称为n-3)长链多不饱和脂肪酸(≥C20；LC-PUFAs)引起了相当大的兴趣，这是基于饮食健康益处和全球来源(鱼油)同时下降的明确证据。产生omega-3 LC-PUFAs的替代转基因植物来源，即二十碳五烯酸(20：5n-3，EPA)和二十二碳六烯酸(22：6n-3，DHA)此前已被证明是有问题的。在这里，我们描述了一组异源基因，它们能够有效地指导这些脂肪酸在作物油茶籽油中的合成，同时避免不需要的中间脂肪酸的积累。我们描述了两个迭代：RRes_EPA，其中种子含有高达31%(平均24%)的EPA水平，以及RRes_DHA，其中种子积累高达12%EPA和14%DHA(平均11%EPA和8%DHA)。这些omega-3 LC-PUFA水平相当于鱼油中的水平，代表了这些脂肪酸的可持续陆地来源。我们还描述了这些非天然脂肪酸在南瓜种子脂中的分布，并在我们目前对种子油合成过程中的酰基交换的理解的背景下考虑了这些数据。
Omega-3 (also called n-3) long-chain polyunsaturated fatty acids (≥C20; LC-PUFAs) are of considerable interest, based on clear evidence of dietary health benefits and the concurrent decline of global sources (fish oils). Generating alternative transgenic plant sources of omega-3 LC-PUFAs, i.e. eicosapentaenoic acid (20:5 n-3, EPA) and docosahexaenoic acid (22:6 n-3, DHA) has previously proved problematic. Here we describe a set of heterologous genes capable of efficiently directing synthesis of these fatty acids in the seed oil of the crop Camelina sativa, while simultaneously avoiding accumulation of undesirable intermediate fatty acids. We describe two iterations: RRes_EPA in which seeds contain EPA levels of up to 31% (mean 24%), and RRes_DHA, in which seeds accumulate up to 12% EPA and 14% DHA (mean 11% EPA and 8% DHA). These omega-3 LC-PUFA levels are equivalent to those in fish oils, and represent a sustainable, terrestrial source of these fatty acids. We also describe the distribution of these non-native fatty acids within C. sativa seed lipids, and consider these data in the context of our current understanding of acyl exchange during seed oil synthesis.
登录
查看更多内容
影响因子：
6.5
作者：
Meyer, A;Kirsch, H;Heinz, E
通讯作者：
Heinz, E
影响因子：
4.1
作者：
Domergue, F;Abbadi, A;Heinz, E
通讯作者：
Heinz, E
影响因子：
8.4
作者：
Ruiz-Lopez, Noemi;Haslam, Richard P.;Usher, Sarah L.;Napier, Johnathan A.;Sayanova, Olga
通讯作者：
Sayanova, Olga
影响因子：
3.8
作者：
Sayanova, Olga;Haslam, Richard P.;Napier, Johnathan A.
通讯作者：
Napier, Johnathan A.
影响因子：
4.8
作者：
Hoffmann, Mareike;Wagner, Martin;Feussner, Ivo
通讯作者：
Feussner, Ivo