Proteomic identification of dengue virus binding proteins in Aedes aegypti mosquitoes and Aedes albopictus cells.
Proteomic identification of dengue virus binding proteins in Aedes aegypti mosquitoes and Aedes albopictus cells.
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DOI：
10.1155/2013/875958
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发表时间：
2013
影响因子：
--
通讯作者：
Black WC 4th
中科院分区：
文献类型：
--
作者：
Muñoz Mde L;Limón-Camacho G;Tovar R;Diaz-Badillo A;Mendoza-Hernández G;Black WC 4th
在美国，登革热的主要传播媒介是埃及伊蚊，登革热病毒通过中肠上皮细胞的受体感染埃及伊蚊。登革病毒的包膜蛋白（E）通过其结构域III与宿主细胞上存在的受体结合，该结构域III主要被认为与细胞受体结合。为了鉴定潜在的受体，使用具有与琼脂糖凝胶4 B共价结合的重组E蛋白结构域III（rE-DIII）或DENV颗粒的柱通过亲和纯化来自蚊子中肠组织和C6/36细胞的蛋白质，以比较和评估它们结合蛋白质（包括来自Ae的雌性蚊子的推定受体）的性能。埃及人。为了确定它们的身份，对通过聚丙烯酰胺凝胶电泳分离的纯化蛋白进行质谱分析。我们的研究结果表明，病毒颗粒和rE-DIII结合蛋白具有相同的表观分子量为57和67 kDa。此外，病毒颗粒结合高分子量蛋白质。纯化的蛋白质鉴定烯醇化酶，β-肾上腺素能受体激酶（β-ARK），翻译延伸因子EF-1 α/Tu，和钙粘蛋白。
The main vector of dengue in America is the mosquito Aedes aegypti, which is infected by dengue virus (DENV) through receptors of midgut epithelial cells. The envelope protein (E) of dengue virus binds to receptors present on the host cells through its domain III that has been primarily recognized to bind cell receptors. In order to identify potential receptors, proteins from mosquito midgut tissue and C6/36 cells were purified by affinity using columns with the recombinant E protein domain III (rE-DIII) or DENV particles bound covalently to Sepharose 4B to compare and evaluate their performance to bind proteins including putative receptors from female mosquitoes of Ae. aegypti. To determine their identity mass spectrometric analysis of purified proteins separated by polyacrylamide gel electrophoresis was performed. Our results indicate that both viral particles and rE-DIII bound proteins with the same apparent molecular weights of 57 and 67 kDa. In addition, viral particles bound high molecular weight proteins. Purified proteins identified were enolase, beta-adrenergic receptor kinase (beta-ARK), translation elongation factor EF-1 alpha/Tu, and cadherin.
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影响因子：
2.9
作者：
Huang, Kuo-Chun;Lee, Ming-Che;Cheng, Jya-Wei
通讯作者：
Cheng, Jya-Wei
影响因子：
13.5
作者：
Hilgard, P;Stockert, R
通讯作者：
Stockert, R
DOI：
10.1016/j.bbrc.2003.08.053
发表时间：
2003-09-26
影响因子：
3.1
作者：
Chiu, MW;Yang, YL
通讯作者：
Yang, YL
影响因子：
5
作者：
Huerta, Vivian;Chinea, Glay;Padron, Gabriel
通讯作者：
Padron, Gabriel
影响因子：
3.8
作者：
GOLLINS, SW;PORTERFIELD, JS
通讯作者：
PORTERFIELD, JS