Immunohistochemical and nucleic acid analysis of somatotropin receptor populations in the bovine ovary.
Immunohistochemical and nucleic acid analysis of somatotropin receptor populations in the bovine ovary.
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牛卵巢中生长激素受体群体的免疫组织化学和核酸分析。
DOI：
10.1095/biolreprod48.6.1219
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发表时间：
1993
影响因子：
3.6
通讯作者：
G. Krivi
中科院分区：
文献类型：
--
作者：
M. Lucy;Robert J Collier;M. Kitchell;J. Dibner;Scott D. Hauser;G. Krivi
通过免疫组织化学、溶液杂交/核酸酶保护、北方印迹和逆转录酶聚合酶链反应（RT-PCR）分析卵巢生长激素受体蛋白和mRNA。免疫过氧化物酶染色显示，CL表达免疫反应性生长激素受体（阳性染色）。卵巢基质、结缔组织、内皮细胞和红细胞不表达生长激素受体（阴性染色）。在CL中，生长激素受体蛋白主要在大的黄体细胞中表达，而小的黄体细胞为阴性。大多数卵泡（1-5 mm，固定后）生长激素受体阴性。在溶液杂交/核酸酶保护的基础上，生长激素受体的mRNA在CL和大黄体细胞中最丰富，在小黄体细胞或卵泡中几乎检测不到（1类，3-5 mm; 2类，6-9 mm; 3类，>或= 10 mm）。生长激素受体mRNA的北方印迹显示生长激素受体mRNA转录本在整个卵巢（4.7和4.4 kb）、CL（4.7和4.4 kb）和肝脏（4.4 kb）中表达; RT-PCR扩增了CL和肝脏中生长激素受体的单个氨基酸编码区。总之，生长激素受体（免疫反应蛋白和mRNA）主要存在于大的黄体细胞中，在卵泡中发现少量表达的受体或其信息。生长激素受体mRNA的替代大小表明牛卵巢中的新mRNA加工。
Ovaries were analyzed for somatotropin receptor protein and mRNA through use of immunohistochemistry, solution hybridization/nuclease protection, Northern blotting, and reverse transcriptase polymerase chain reaction (RT-PCR). As indicated by immunoperoxidase staining, CL expressed immunoreactive somatotropin receptor (positive stain). Ovarian stroma, connective tissue, endothelium, and erythrocytes did not express somatotropin receptor (negative stain). Within the CL, somatotropin receptor protein was expressed primarily in large luteal cells whereas small luteal cells were negative. Most follicles (1-5 mm, after fixation) were negative for somatotropin receptor. On the basis of solution hybridization/nuclease protection, the mRNA for somatotropin receptor was found in greatest abundance in CL and large luteal cells and was nearly undetectable in small luteal cells or follicles (class 1, 3-5 mm; class 2, 6-9 mm; and class 3, > or = 10 mm). Northern blotting of mRNA for somatotropin receptor showed expression of somatotropin receptor mRNA transcripts in whole ovary (4.7 and 4.4 kb), CL (4.7 and 4.4 kb), and liver (4.4 kb); and RT-PCR amplified a single amino acid coding region for somatotropin receptor in CL and liver. In summary, somatotropin receptor (both immunoreactive protein and mRNA) is found primarily in the large luteal cell, and lesser amounts of the expressed receptor or its message are found in the follicle. Alternative sizes of mRNA for somatotropin receptor suggest novel mRNA processing in the bovine ovary.
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DOI：
10.1210/mend.6.2.1569971
发表时间：
1992
期刊：
Molecular endocrinology (Baltimore, Md.)
影响因子：
--
作者：
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1073/pnas.86.20.8083
发表时间：
1989-10-01
影响因子：
11.1
作者：
GODOWSKI, PJ;LEUNG, DW;WOOD, WI
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1073/pnas.81.3.935
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DERCOLE, AJ;STILES, AD;UNDERWOOD, LE
通讯作者：
UNDERWOOD, LE