Immunological analysis of chick notochord and cartilage matrix development with antisera to cartilage matrix macromolecules.
Immunological analysis of chick notochord and cartilage matrix development with antisera to cartilage matrix macromolecules.
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用软骨基质大分子抗血清对鸡脊索和软骨基质发育进行免疫学分析。
DOI：
10.1016/0012-1606(85)90346-x
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发表时间：
1985
影响因子：
2.7
通讯作者：
Pacifici,M
中科院分区：
文献类型：
--
作者：
Oettinger,HF;Thal,G;Sasse,J;Holtzer,H;Pacifici,M
用软骨主要细胞外基质成分的抗体对9-16期鸡胚头后区和17-31期鸡胚翅芽区的横向冷冻切片进行染色。这些探针包括未分级的A1和A2抗血清的主要软骨蛋白多糖，亲和纯化的抗体的蛋白多糖核心蛋白和II型胶原蛋白，和硫酸角质素的单克隆抗体。早在第10阶段的胚胎中，脊索就被硫酸角质素单克隆抗体特异性染色。在这个阶段，脊索，以及周围组织，软骨蛋白多糖和胶原抗体呈阴性。后一种探针的阳性染色由脊索细胞及其伴随的鞘在第15期周围协调获得，而周围组织保持阴性。在这个阶段，脊髓周围鞘的腹侧区域表现出与蛋白多糖和硫酸角质素抗体的浅染色，但未能与II型胶原抗体反应。阳性染色的脊索和腹侧脊髓持续到以后的发展阶段。正如免疫荧光所揭示的那样，最终的脊椎成软骨细胞首先出现在大约23期，最终的肢体成软骨细胞出现在25期。结果进行了讨论，在早期胚胎发育中的脊索可能的多种作用。
Transverse frozen sections from the postcephalic region of stage 9–16 chick embryos and from the wing bud region of stage 17–31 embryos were stained with antibodies to the major extracellular matrix components of cartilage. These probes included unfractionated A1 and A2 antisera to the major cartilage proteoglycan, affinity-purified antibodies to the proteoglycan core protein and to Type II collagen, and a monoclonal antibody to keratan sulfate. In embryos as early as stage 10, notochord stained specifically with the keratan sulfate monoclonal antibody. At this stage the notochord, as well as surrounding tissues, were negative to cartilage proteoglycan and collagen antibodies. Positive staining with the latter probes was coordinately acquired by notochord cells and their accompanying sheath around stage 15, while surrounding tissues remained negative. At this stage, the ventral region of the perispinal cord sheath exhibited light staining with the proteoglycan and keratan sulfate antibodies though failing to react to Type II collagen antibodies. Positive staining of notochord and ventral spinal cord persisted through later developmental stages. As revealed by immunofluorescence, definitive vertebral chondroblasts first emerged at approximately stage 23 and definitive limb chondroblasts at stage 25. The results are discussed in terms of the possible multiple roles of notochord in early embryogenesis.
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苯环己哌啶类药物抑制大鼠纹状体中兴奋性氨基酸诱导的神经递质释放的表征。
DOI：
--
发表时间：
1986
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Snell,LD;Johnson,KM
通讯作者：
Johnson,KM
影响因子：
2.5
作者：
HONEY, CR;MILJKOVIC, Z;MACDONALD, JF
通讯作者：
MACDONALD, JF
[3H]TCP 结合定位与 NMDA 受体之间高度相关。
影响因子：
5
作者：
Maragos,WF;Chu,DC;Greenamyre,JT;Penney,JB;Young,AB
通讯作者：
Young,AB
影响因子：
64.8
作者：
JOHNSON, JW;ASCHER, P
通讯作者：
ASCHER, P
放射性配体与中心苯环己哌啶识别位点的结合依赖于兴奋性氨基酸受体激动剂。
影响因子：
5
作者：
P. Loo;A. Braunwalder;J. Lehmann;M. Williams
通讯作者：
M. Williams