Cathepsin K-Cre causes unexpected germline deletion of genes in mice.
Cathepsin K-Cre causes unexpected germline deletion of genes in mice.
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DOI：
10.1371/journal.pone.0042005
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Weber JD
中科院分区：
文献类型：
--
作者：
Winkeler CL;Kladney RD;Maggi LB Jr;Weber JD
Osteoclasts are terminally differentiated cells that attach to bone and secrete proteases to degrade the bone matrix. The primary protease responsible for the degradation of the organic component of the bone matrix is Cathepsin K, which was largely thought to be unique to osteoclasts. Given its apparent selective expression in osteoclasts, the Cathepsin K promoter has been engineered to drive the expression of Cre recombinase in mice and has been the most relevant tool for generating osteoclast-specific gene loss. In an effort to understand the role of the ARF tumor suppressor in osteoclasts, we crossed Arf fl/fl mice to CtskCre/+ mice, which unexpectedly resulted in the germline loss of Arf. We subsequently confirmed Cre activity in gametes by generating CtskCre/+; Rosa+ mice. These results raise significant concerns regarding in vivo bone phenotypes created using CtskCre/+ mice and warrant further investigation into the role of Cathepsin K in gametes as well as alternative tools for studying osteoclast-specific gene loss in vivo.
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影响因子：
5.3
作者：
Apicelli, Anthony J.;Maggi, Leonard B., Jr.;Weber, Jason D.
通讯作者：
Weber, Jason D.
DOI：
10.1073/pnas.0902310106
发表时间：
2009-04-14
影响因子：
11.1
作者：
Gromley, Adam;Churchman, Michelle L.;Sherr, Charles J.
通讯作者：
Sherr, Charles J.
DOI：
10.1515/bchm3.1995.376.6.379
发表时间：
1995-06-01
期刊：
BIOLOGICAL CHEMISTRY HOPPE-SEYLER
影响因子：
--
作者：
BROMME, D;OKAMOTO, K
通讯作者：
OKAMOTO, K
DOI：
10.1073/pnas.95.23.13453
发表时间：
1998-11-10
影响因子：
11.1
作者：
Saftig, P;Hunziker, E;von Figura, K
通讯作者：
von Figura, K
影响因子：
3.5
作者：
Rantakokko, J;Aro, HT;Vuorio, E
通讯作者：
Vuorio, E