Ablation of Dicer from murine Schwann cells increases their proliferation while blocking myelination.
Ablation of Dicer from murine Schwann cells increases their proliferation while blocking myelination.
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DOI：
10.1371/journal.pone.0012450
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发表时间：
2010-08-27
期刊：
影响因子：
3.7
通讯作者：
Aguzzi A
中科院分区：
文献类型：
--
作者：
Bremer J;O'Connor T;Tiberi C;Rehrauer H;Weis J;Aguzzi A
周围神经系统的粗轴突周围的髓鞘是由高度特化的许旺细胞产生的。雪旺细胞的分化和髓鞘形成以不连续的步骤发生。每一个都需要特定蛋白质以精确的序列协调表达，但在这些事件中控制蛋白质表达的调控机制还不完全清楚。在这里，我们报告说，雪旺细胞特异性消融酶Dicer 1，这是生产小的非编码调节microRNA所需的，完全逮捕雪旺细胞分化，导致出生后早期死亡。Dicer−/− Schwann细胞失去了髓鞘形成的能力，但仍然能够对轴突进行分类。细胞死亡和，矛盾的是，未成熟的雪旺细胞的增殖显着增强，这表明它们的终末分化是由生长停滞调节microRNA触发的。使用microRNA微阵列，我们鉴定了16种在髓鞘形成后上调的microRNA，其表达由Schwann细胞中的Dicer控制。这组microRNA似乎驱动许旺细胞分化和周围神经的髓鞘形成，从而实现生物体生存的关键功能。
The myelin sheaths that surround the thick axons of the peripheral nervous system are produced by the highly specialized Schwann cells. Differentiation of Schwann cells and myelination occur in discrete steps. Each of these requires coordinated expression of specific proteins in a precise sequence, yet the regulatory mechanisms controlling protein expression during these events are incompletely understood. Here we report that Schwann cell-specific ablation of the enzyme Dicer1, which is required for the production of small non-coding regulatory microRNAs, fully arrests Schwann cell differentiation, resulting in early postnatal lethality. Dicer−/− Schwann cells had lost their ability to myelinate, yet were still capable of sorting axons. Both cell death and, paradoxically, proliferation of immature Schwann cells was markedly enhanced, suggesting that their terminal differentiation is triggered by growth-arresting regulatory microRNAs. Using microRNA microarrays, we identified 16 microRNAs that are upregulated upon myelination and whose expression is controlled by Dicer in Schwann cells. This set of microRNAs appears to drive Schwann cell differentiation and myelination of peripheral nerves, thereby fulfilling a crucial function for survival of the organism.
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DOI：
10.4161/cc.9.6.10987
发表时间：
2010-03-15
期刊：
Cell cycle (Georgetown, Tex.)
影响因子：
--
作者：
Guessous F;Zhang Y;Kofman A;Catania A;Li Y;Schiff D;Purow B;Abounader R
通讯作者：
Abounader R
DOI：
10.1073/pnas.0501196102
发表时间：
2005-06-28
影响因子：
11.1
作者：
Tapinos, N;Rambukkana, A
通讯作者：
Rambukkana, A
影响因子：
1.5
作者：
Naiche, L. A.;Papaioannou, Virginia E.
通讯作者：
Papaioannou, Virginia E.
影响因子：
5.3
作者：
Davis, Tigwa H.;Cuellar, Trinna L.;Ullian, Erik M.
通讯作者：
Ullian, Erik M.
影响因子：
11.4
作者：
RIDLEY, AJ;PATERSON, HF;LAND, H
通讯作者：
LAND, H