Intracellular signaling by diffusion: can waves of hydrogen peroxide transmit intracellular information in plant cells?
Intracellular signaling by diffusion: can waves of hydrogen peroxide transmit intracellular information in plant cells?
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DOI：
10.3389/fpls.2012.00295
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发表时间：
2012
影响因子：
5.6
通讯作者：
Møller IM
中科院分区：
文献类型：
--
作者：
Vestergaard CL;Flyvbjerg H;Møller IM
Ca2+ 离子的调幅和调频波在细胞内传输信息。活性氧（ROS），特别是过氧化氢，被认为在植物细胞中具有类似的作用。鉴于植物细胞的物理和生化条件，我们考虑了这种细胞内通讯系统的可行性。作为模型系统，我们使用源自质膜 (PM) 并通过细胞质传播的 H2O2 信号。我们考虑两种最简单的信号类型：孤立脉冲和谐波振荡。首先，我们考虑此类信号的基本限制，如信号源、频率、幅度和距离。然后我们确定了 ROS 去除酶对 H2O2 传递信号能力的影响。最后，我们考虑细胞质流在多大程度上扭曲了信号。该模型使我们能够预测扩散介导的信号传导可能发生的条件。我们证明，在远高于 1Hz（这是实验观察到的最高频率）的频率下，H2O2 在 1μm 距离（细胞器之间的典型距离，可以充当中继站）上进行纯粹的细胞内信息扩散传输是可能的。这允许信号的频率和幅度调制。在 10μm 的距离（PM 和原子核之间的典型距离）上发送信号是可能的，但需要高信号幅度或相当低的检测阈值。此外，在如此长的距离上，需要高速率的酶降解才能使频率高于 0.1Hz 的信号成为可能。在任何一种情况下，细胞质流都不会严重干扰信号。我们的结论是，虽然没有中继站的纯粹扩散介导的信号传导在理论上是可能的，但在实践中不太可能起作用，因为它需要比实验观察到的更快的酶促降解和更低的细胞背景 H2O2 浓度。
Amplitude- and frequency-modulated waves of Ca2+ ions transmit information inside cells. Reactive Oxygen Species (ROS), specifically hydrogen peroxide, have been proposed to have a similar role in plant cells. We consider the feasibility of such an intracellular communication system in view of the physical and biochemical conditions in plant cells. As model system, we use a H2O2 signal originating at the plasma membrane (PM) and spreading through the cytosol. We consider two maximally simple types of signals, isolated pulses and harmonic oscillations. First we consider the basic limits on such signals as regards signal origin, frequency, amplitude, and distance. Then we establish the impact of ROS-removing enzymes on the ability of H2O2 to transmit signals. Finally, we consider to what extent cytoplasmic streaming distorts signals. This modeling allows us to predict the conditions under which diffusion-mediated signaling is possible. We show that purely diffusive transmission of intracellular information by H2O2 over a distance of 1 μm (typical distance between organelles, which may function as relay stations) is possible at frequencies well above 1 Hz, which is the highest frequency observed experimentally. This allows both frequency and amplitude modulation of the signal. Signaling over a distance of 10 μm (typical distance between the PM and the nucleus) may be possible, but requires high signal amplitudes or, equivalently, a very low detection threshold. Furthermore, at this longer distance a high rate of enzymatic degradation is required to make signaling at frequencies above 0.1 Hz possible. In either case, cytoplasmic streaming does not seriously disturb signals. We conclude that although purely diffusion-mediated signaling without relaying stations is theoretically possible, it is unlikely to work in practice, since it requires a much faster enzymatic degradation and a much lower cellular background concentration of H2O2 than observed experimentally.
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影响因子：
4.8
作者：
Bienert, Gerd P.;Moller, Anders L. B.;Jahn, Thomas P.
通讯作者：
Jahn, Thomas P.
影响因子：
4
作者：
JAFFE, LF
通讯作者：
JAFFE, LF
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影响因子：
11.6
作者：
Li, Xiaojuan;Wang, Xiaohua;Lin, Jinxing
通讯作者：
Lin, Jinxing
DOI：
10.1073/pnas.96.14.8271
发表时间：
1999-07-06
影响因子：
11.1
作者：
Maxwell, DP;Wang, Y;McIntosh, L
通讯作者：
McIntosh, L
影响因子：
6.4
作者：
Foyer, CH;Noctor, G
通讯作者：
Noctor, G