Effects of temperature and analyte application technique on neuron-based chemical sensing.
Effects of temperature and analyte application technique on neuron-based chemical sensing.
复制标题
温度和分析物应用技术对基于神经元的化学传感的影响。
DOI：
10.1016/0956-5663(92)90014-e
复制
发表时间：
1992
影响因子：
12.6
通讯作者：
Barnes,CD
中科院分区：
文献类型：
--
作者：
Skeen,RS;VanWie,BJ;Fung,SJ;Barnes,CD
结果提出了增强基于神经元的传感领域提供洞察的工作温度和分析物的应用技术（脉冲与返混）对传感性能的影响。在这些研究中，测量了来自池塘蜗牛的巨大内脏神经元VV 1和VV 2的5-羟色胺感受属性。使用快速流体交换系统的实验揭示了最大发射频率的浓度依赖性增加，类似于先前报道的缓慢混合应用。然而，在快速应用的情况下，更快地达到最大激发频率，并且在最大响应和灵敏度两者中存在较少的细胞间变异性。给定应用技术，温度的增加导致灵敏度和最大激发频率的增加，以及在去除分析物之后响应返回到基线所需的时间的减少。为了提供对阿托宁诱导的反应的动力学的见解，详细检查了温度和浓度对活化、恢复和脱敏速率的影响。一般来说，发现温度的增加增加了激活和脱敏的速率，而对恢复的影响不明显。此外，激活和脱敏的速率都与浓度成正相关，而恢复速率则与浓度成负相关。
Results are presented which enhance the field of neuron-based sensing by providing insight on the effects of operating temperature and analyte application technique (pulse versus back-mixed) on sensing properties. In these studies, serotonin sensing attributes of giant visceral neurons VV1 and VV2 from the pond snailLymnea stagnaliswere measured. Experiments using a rapid fluid-exchange system reveal a concentration-dependent increase in maximum firing frequency similar to that reported earlier for a slow well-mixed application. With a rapid application, however, the maximum firing frequency is reached more quickly, and there is less cell-to-cell variability in both the maximum response and sensitivity. Given an application technique, an increase in temperature causes an increase in sensitivity and maximum firing frequency, as well as a decrease in the time required for the response to return to baseline following removal of the analyte. To provide insights on the kinetics of the serotonin-induced response, the effects of temperature and concentration on the rates of activation, recovery and desensitization were examined in detail. In general, it was found that an increase in temperature increases the rates of activation and desensitization, while the effects on recovery were not apparent. In addition, both the rates of activation and desensitization have a direct dependence on concentration while the rate of recovery has an inverse dependence.
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DOI：
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发表时间：
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期刊：
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