Influence of the Two-Layer Preservation Method on Human Pancreatic Islet Isolation: A Meta-Analysis
Influence of the Two-Layer Preservation Method on Human Pancreatic Islet Isolation: A Meta-Analysis
复制标题
两层保存方法对人胰岛分离的影响：荟萃分析
DOI：
10.5301/ijao.5000391
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发表时间：
2015-03
影响因子：
1.7
通讯作者：
Dong Ming
中科院分区：
文献类型：
--
作者：
Li Xiaohang;Zhang Jialin;Sang Lixuan;Chu Zhiqiang;Dong Ming
引言在胰岛分离之前，关于双层方法（TLM）是否上级于威斯康星州大学溶液（UW）用于保存人胰腺一直存在争议。目前荟萃分析的目的是评估哪种方法更上级。方法我们检索电子数据库（MEDLINE、EMBASE、科克伦对照试验中心注册库），以查找2000年1月至2013年10月期间以英文发表的相关人体试验。提取供体特征和胰岛分离结果的数据。结果共纳入14篇文献，包括18项人体研究。与单独UW相比，单独TLM产生显著更高的胰岛产量（加权平均差异，776.32; 95%置信区间; 370.82-1181.82; P = .0002）。单独TLM也产生了较高比例的可移植制剂（比值比，1.60; 95%置信区间; 1.15-2.23; P = .005）。以下指标没有差异：胰岛活力（加权平均差异，2.10;-2.41-6.60; P = .360），纯度（加权平均差异，-0.92;-3.75-1.91; P = .520）和通过测量刺激指数评估的功能（加权平均差异，0.17;-0.21-0.55; P = .380）。当比较UW储存后的TLM与单独的UW时，结果与之前的结果相似。结论TLM可提高胰岛产量，增加胰岛移植机会。因此，TLM应推荐用于在胰岛分离之前保存人胰腺。
Introduction There has been continuous debate on whether the Two-Layer Method (TLM) is superior to the University of Wisconsin solution (UW) for preserving human pancreas prior to islet isolation. The objective of the current meta-analysis is to assess which method is superior. Methods We searched electronic databases (MEDLINE, EMBASE, Cochrane Central Register of Controlled Trials) for relevant human trials published in the English language from January 2000 to October 2013. Data on donor characteristics and islet isolation outcomes were extracted. Results 14 articles containing 18 human studies were included in this meta-analysis. In comparison to UW alone, TLM alone produced a significantly higher islet yield (weighted mean difference, 776.32; 95% confidence interval; 370.82-1181.82; P = .0002). TLM alone also yielded higher proportion of transplantable preparations (odds ratio, 1.60; 95% confidence interval; 1.15-2.23; P = .005). The following measures did not differ: islet viability (weighted mean difference, 2.10; −2.41-6.60; P = .360), purity (weighted mean difference, −0.92; −3.75-1.91; P = .520) and function assessed by measuring the stimulation index (weighted mean difference, 0.17; −0.21-0.55; P = .380). When comparing TLM following UW storage with UW alone, the results were similar to the previous ones. Conclusions This data indicates that the TLM can improve islet yield and increase the opportunities of human pancreatic islet transplantation. Therefore, the TLM should be recommended for preserving human pancreas prior to islet isolation.
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影响因子：
3.1
作者：
Y. Iwanaga;Yusuyuki Suzuki;Y. Okada;H. Mori;I. Matsumoto;M. Mitsutsuji;Y. Tanioka;Y. Fujino;M. Tominaga;Y. Ku;Y. Kuroda
通讯作者：
Y. Iwanaga;Yusuyuki Suzuki;Y. Okada;H. Mori;I. Matsumoto;M. Mitsutsuji;Y. Tanioka;Y. Fujino;M. Tominaga;Y. Ku;Y. Kuroda
影响因子：
3.3
作者：
Huanying Qin;S. Matsumoto;G. Klintmalm;E. De Vol
通讯作者：
Huanying Qin;S. Matsumoto;G. Klintmalm;E. De Vol
影响因子：
6.2
作者：
T. Tsujimura;Y. Kuroda;T. Kin;J. Avila;R. Rajotte;G. Korbutt;E. Ryan;A. M. Shapiro;J. Lakey
通讯作者：
T. Tsujimura;Y. Kuroda;T. Kin;J. Avila;R. Rajotte;G. Korbutt;E. Ryan;A. M. Shapiro;J. Lakey
影响因子：
0.9
作者：
T. Tsujimura;T. Tsujimura;Y. Kuroda;Y. Kuroda;J. Avila;J. Avila;T. Kin;T. Kin;T. Churchill;Tom Churchill;A. M. Shapiro;A. M. Shapiro;J. Lakey
通讯作者：
T. Tsujimura;T. Tsujimura;Y. Kuroda;Y. Kuroda;J. Avila;J. Avila;T. Kin;T. Kin;T. Churchill;Tom Churchill;A. M. Shapiro;A. M. Shapiro;J. Lakey
影响因子：
3.1
作者：
Y. Kuroda;Y. Fujino;A. Morita;Y. Tanioka;Y. Suzuki;T. Kawamura;Y. Ku;Y. Saitoh
通讯作者：
Y. Kuroda;Y. Fujino;A. Morita;Y. Tanioka;Y. Suzuki;T. Kawamura;Y. Ku;Y. Saitoh