Plasma membrane microdomains act as concentration platforms to facilitate intoxication by aerolysin.
Plasma membrane microdomains act as concentration platforms to facilitate intoxication by aerolysin.
复制标题
质膜微域充当浓度平台，可促进气氛蛋白中毒。
DOI：
10.1083/jcb.147.1.175
复制
发表时间：
1999-10-04
期刊：
影响因子：
--
通讯作者：
van Der Goot FG
中科院分区：
文献类型：
--
作者：
Abrami L;van Der Goot FG
已有研究表明，许多细胞的质膜中都含有富含胆固醇的鞘磷脂微区。在这里，我们分析了这些微域在促进细菌致孔毒素溶气素齐聚过程中的作用。Aeroly-sin通过糖基磷脂酰肌醇锚定受体与细胞结合，作为一种亲水性可溶性蛋白，必须聚合成两亲性环状复合体才能形成孔。我们首先证明，当活细胞表面的毒素浓度比溶液中的毒素浓度低105倍时，可以发生寡聚反应。我们的观察表明，不仅靶细胞上受体的数量对毒素敏感性很重要，而且它们与耐洗涤剂微域瞬时结合的能力也很重要。寡聚似乎是因为与其糖基磷脂酰肌醇锚定受体结合的毒素可以被招募到这些微域中，作为浓缩装置。
It has been proposed that the plasma membrane of many cell types contains cholesterol-sphingolipid–rich microdomains. Here, we analyze the role of these microdomains in promoting oligomerization of the bacterial pore-forming toxin aerolysin. Aeroly-sin binds to cells, via glycosyl phosphatidylinositol- anchored receptors, as a hydrophilic soluble protein that must polymerize into an amphipathic ring-like complex to form a pore. We first show that oligomerization can occur at >105-fold lower toxin concentration at the surface of living cells than in solution. Our observations indicate that it is not merely the number of receptors on the target cell that is important for toxin sensitivity, but their ability to associate transiently with detergent resistant microdomains. Oligomerization appears to be promoted by the fact that the toxin bound to its glycosyl phosphatidylinositol-anchored receptors, can be recruited into these microdomains, which act as concentration devices.
登录
查看更多内容
影响因子：
7.8
作者：
Abrami, L;Fivaz, M;Glauser, PE;Parton, RG;van der Goot, FG
通讯作者：
van der Goot, FG
影响因子：
3.6
作者：
Cowell, S;Aschauer, W;Buckley, JT
通讯作者：
Buckley, JT
影响因子：
7.5
作者：
Harder, T;Simons, K
通讯作者：
Simons, K
影响因子：
4.8
作者：
HANADA, K;NISHIJIMA, M;PAGANO, RE
通讯作者：
PAGANO, RE
影响因子：
9.2
作者：
Hooper, NM
通讯作者：
Hooper, NM