Construction of Immunoglobulin Fusion Proteins
Construction of Immunoglobulin Fusion Proteins
复制标题
免疫球蛋白融合蛋白的构建
DOI:
10.1002/0471142735.im1019as48
复制
发表时间:
2002
影响因子:
--
通讯作者:
A. Aruffo
中科院分区:
文献类型:
--
作者:
D. Hollenbaugh;A. Aruffo
Recombinant DNA technology has allowed the preparation of chimeric genes encoding proteins with novel properties. This unit describes the construction and subsequent testing of genes encoding immunoglobulin chimeras. The first protocol details fusion of a protein (or protein fragment) of interest onto an immunoglobulin constant region using a modified version of the expression vector pCDM8. The resulting fusion protein generally retains the functional properties of both the protein of interest and the immunoglobulin constant region; this can be demonstrated as described here.
影响因子:
56.9
作者:
WALZ, G;ARUFFO, A;SEED, B
通讯作者:
SEED, B