Construction of Immunoglobulin Fusion Proteins

Construction of Immunoglobulin Fusion Proteins
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免疫球蛋白融合蛋白的构建

DOI:
10.1002/0471142735.im1019as48
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发表时间:
2002
影响因子:
--
通讯作者:
A. Aruffo
A. Aruffo
中科院分区:
--
文献类型:
--
作者:
D. Hollenbaugh;A. Aruffo

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重组DNA技术使编码具有新特性的蛋白质的嵌合基因的制备成为可能。本单元描述了编码免疫球蛋白嵌合体的基因的构建和随后的测试。第一个方案详细说明了使用表达载体pCDM8的修改版本将感兴趣的蛋白质(或蛋白质片段)融合到免疫球蛋白恒定区上。得到的融合蛋白通常保留了目的蛋白和免疫球蛋白恒定区的功能性质;这可以如这里所描述的那样得到证明。
Recombinant DNA technology has allowed the preparation of chimeric genes encoding proteins with novel properties. This unit describes the construction and subsequent testing of genes encoding immunoglobulin chimeras. The first protocol details fusion of a protein (or protein fragment) of interest onto an immunoglobulin constant region using a modified version of the expression vector pCDM8. The resulting fusion protein generally retains the functional properties of both the protein of interest and the immunoglobulin constant region; this can be demonstrated as described here.
DOI: 10.1126/science.1701275
发表时间: 1990-11-23
期刊: SCIENCE
影响因子: 56.9
作者:
WALZ, G;ARUFFO, A;SEED, B
通讯作者: SEED, B