In vitro mineralization of dense collagen substrates: a biomimetic approach toward the development of bone-graft materials.
In vitro mineralization of dense collagen substrates: a biomimetic approach toward the development of bone-graft materials.
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DOI：
10.1016/j.actbio.2011.04.014
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发表时间：
2011-08
影响因子：
9.7
通讯作者：
Gower, Laurie B.
中科院分区：
文献类型：
--
作者：
Thula, Taili T.;Rodriguez, Douglas E.;Lee, Myong Hwa;Pendi, Laura;Podschun, Jacob;Gower, Laurie B.
骨是一种有机-无机复合材料，具有层次化结构，具有高强度和高韧性。骨的纳米结构由嵌入并排列在胶原纤维间隙中的纳米羟基磷灰石晶体组成。这种独特的纳米结构导致了优异的机械和生物性能，使得在没有类似骨的纳米结构材料的情况下很难模拟骨的特性。我们小组工作的一个主要目标是使用仿生处理技术来产生类似骨骼的结构。在我们以前的研究中，我们证明了多孔胶原海绵的纤维内矿化可以通过聚合物诱导的液体前体(PILP)矿化过程实现，从而形成类似骨的纳米结构。这项研究的目的是研究使用这种聚合物导向的结晶过程来矿化致密的胶原基质。为了检测真正代表致密骨基质的胶原支架，对海牛骨进行了脱矿处理，以分离其胶原基质，该基质由致密的片状骨微结构组成。然后使用聚天冬氨酸通过无定形前体途径引导矿化过程，对这种生物胶原支架进行再矿化。考察了聚合物相对分子质量、底物尺寸和矿化时间等条件。该工艺的矿物渗透深度可达100μms，而传统的结晶工艺只观察到表面沉淀物，没有矿物渗透。用电子显微镜、广角X射线衍射仪和热分析对所制备的羟基磷灰石/胶原蛋白复合材料进行了表征。这些研究表明，使用PILP工艺可以在生物基质中恢复原始的互穿骨纳米结构和骨微结构。
Bone is an organic-inorganic composite which has hierarchical structuring that leads to high strength and toughness. The nanostructure of bone consists of nanocrystals of hydroxyapatite embedded and aligned within the interstices of collagen fibrils. This unique nanostructure leads to exceptional properties, both mechanical and biological, making it difficult to emulate bone properties without having a bone-like nanostructured material. A primary goal of our group’s work is to use biomimetic processing techniques that lead to bone-like structures. In our prior studies, we demonstrated that intrafibrillar mineralization of porous collagen sponges, leading to a bone-like nanostructure, can be achieved using a polymer-induced liquid-precursor (PILP) mineralization process. The objective of this study was to investigate the use of this polymer-directed crystallization process to mineralize dense collagen substrates. To examine collagen scaffolds that truly represent the dense-packed matrix of bone, manatee bone was demineralized to isolate its collagen matrix, consisting of a dense, lamellar osteonal microstructure. This biogenic collagen scaffold was then remineralized using polyaspartate to direct the mineralization process through an amorphous precursor pathway. Various conditions investigated included polymer molecular weight, substrate dimension and mineralization time. Mineral penetration depths of up to 100 μms were achieved using this PILP process, compared to no penetration with only surface precipitates observed for the conventional crystallization process. Electron microscopy, wide-angle X-ray diffraction, and thermal analysis were used to characterize the resulting hydroxyapatite/collagen composites. These studies demonstrate that the original interpenetrating bone nanostructure and osteonal microstructure could be recovered in a biogenic matrix using the PILP process.
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影响因子：
3.8
作者：
Deshpande, Atul S.;Beniash, Elia
通讯作者：
Beniash, Elia
DOI：
10.1098/rspb.2000.1337
发表时间：
2001-01-07
影响因子：
4.7
作者：
Currey, JD;Zioupos, P;Casinos, A
通讯作者：
Casinos, A
影响因子：
62.1
作者：
Gower, Laurie B.
通讯作者：
Gower, Laurie B.
DOI：
10.1016/0141-8130(91)90058-3
发表时间：
1991-04-01
影响因子：
8.2
作者：
BIGI, A;RIPAMONTI, A;KOCH, MHJ
通讯作者：
KOCH, MHJ
影响因子：
2.9
作者：
HELSETH, DL;LECHNER, JH;VEIS, A
通讯作者：
VEIS, A