Copy number variation of CBF-A14 at the Fr-A2 locus determines frost tolerance in winter durum wheat
Copy number variation of CBF-A14 at the Fr-A2 locus determines frost tolerance in winter durum wheat
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Fr-A2基因座CBF-A14的拷贝数变异决定了冬季硬粒小麦的抗冻性
DOI：
10.1007/s00122-016-2685-3
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发表时间：
2016
影响因子：
5.4
通讯作者：
T. Würschum
中科院分区：
文献类型：
--
作者：
Sieber;C.F.H. Longin;W.L. Leiser;T. Würschum
关键信息硬粒小麦的抗冻性主要受Fr-A2位点CBF-A14基因拷贝数变异的控制摘要硬粒小麦抗冻性是硬粒小麦育种成功的关键性状，它可以提高秋播冬粮地区的产量表现。本研究旨在探讨冬性硬粒小麦抗冻性的遗传结构，为冬性硬粒小麦的育种提供分子基础。为此，一个不同的面板的170个冬季和14个春季硬粒小麦基因型的世界各地的起源进行了评估耐霜冻性在该领域，以及在半控制测试。通过测序基因分型方法获得的总共30，611个多态性全基因组标记和候选位点的标记用于评估标记-性状关联。在5A染色体上检测到一个主效QTL，可能对应于抗冻性A2（Fr-A2）。进一步的分析有力地支持了CBF-A14在Fr-A2位点的拷贝数变异是该主效QTL的因果多态性的结论。它解释了91.6%的基因型方差，并且QTL区域中的两个强相关标记的单块也允许捕获该QTL的方差。除了这个主效QTL外，Fr-B2的贡献要小得多，为4.2%。进一步研究发现CBF-A14基因的拷贝数和耐霜冻单倍型的频率反映了基因型来源国的气候条件，表明通过育种进行选择。本研究开发了两个功能性KASP标记，可用于高通量筛选单块小麦，从而进行基于分子标记的冬硬粒小麦抗冻性育种。
Key messageFrost tolerance in durum wheat is mainly controlled by copy number variation ofCBF-A14at theFr-A2locus.AbstractFrost tolerance is a key trait for successful breeding of winter durum wheat (Triticum durum) which can increase the yield performance in regions favoring autumn-sown winter cereals. The aim of this study was to investigate the genetic architecture of frost tolerance in order to provide molecular support for the breeding of winter durum wheat. To this end, a diverse panel of 170 winter and 14 spring durum wheat genotypes of worldwide origin was evaluated for frost tolerance in the field, as well as in a semi-controlled test. A total of 30,611 polymorphic genome-wide markers obtained by a genotyping-by-sequencing approach and markers for candidate loci were used to assess marker-trait associations. One major QTL was detected on chromosome 5A, likely corresponding toFrost Resistance-A2(Fr-A2). Further analyses strongly support the conclusion that copy number variation ofCBF-A14at theFr-A2locus is the causal polymorphism underlying this major QTL. It explains 91.6 % of the genotypic variance and a haploblock of two strongly associated markers in the QTL region also allowed to capture the variance of this QTL. In addition to this major QTL, a much smaller contribution of 4.2 % was observed forFr-B2. We further investigated this major QTL and found that the copy number ofCBF-A14and the frequency of the frost tolerant haplotype mirrored the climatic conditions in the genotypes’ country of origin, suggesting selection through breeding. Two functional KASP markers were developed which facilitate a high-throughput screening of the haploblock and thus a marker-based breeding of frost tolerance in winter durum wheat.
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半对照试验作为硬粒小麦（Triticum durum）抗冻性选择工具的评估
影响因子：
2
作者：
Sieber;T. Würschum;C.F.H. Longin
通讯作者：
C.F.H. Longin
DOI：
10.1073/pnas.1635053100
发表时间：
2003-10-28
影响因子：
11.1
作者：
Trevaskis, B;Bagnall, DJ;Dennis, ES
通讯作者：
Dennis, ES
影响因子：
30.8
作者：
Yu, JM;Pressoir, G;Buckler, ES
通讯作者：
Buckler, ES
影响因子：
2
作者：
Longin, Carl Friedrich Horst;Sieber, Alisa-Naomi;Reif, Jochen C.
通讯作者：
Reif, Jochen C.
影响因子：
1.9
作者：
J. Sutka;J. Snape
通讯作者：
J. Snape