Effect of high carbon dioxide treatment on reactive oxygen species accumulation and antioxidant capacity in fresh-cut pear fruit during storage
Effect of high carbon dioxide treatment on reactive oxygen species accumulation and antioxidant capacity in fresh-cut pear fruit during storage
复制标题
高二氧化碳处理对鲜切梨贮藏过程中活性氧积累及抗氧化能力的影响
DOI：
10.1016/j.scienta.2021.109925
复制
发表时间：
2021-04
影响因子：
4.3
通讯作者：
Zisheng Luo
中科院分区：
文献类型：
--
作者：
Di Wang;Wenxuan Li;Dong Li;Li Li;Zisheng Luo
鲜切梨因其营养、新鲜、方便而广受欢迎。然而，由创伤引起的氧化应激加剧了质量损失。研究了10%CO2对鲜切梨活性氧代谢和抗氧化能力的影响。结果表明，10%CO2处理能有效抑制贮藏过程中ROS的积累，降低过氧化氢（H2O2）和超氧阴离子自由基（O2−）的含量。贮藏结束时，CO2处理组的自由基清除能力（DPPH）、总抗氧化能力（ABTS）和亚铁还原能力（FRAP）分别提高了5.5%、23%和27%。另外，10%CO2处理提高了过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPX）、抗坏血酸过氧化物酶（APX）、谷胱甘肽还原酶（GR）、超氧化物歧化酶（SOD）和过氧化物酶（POD）的活性和基因表达水平，而抑制了NADPH氧化酶（NOX）。抗坏血酸（阿萨）和还原型谷胱甘肽（GSH）的积累也被CO2加速。本研究表明，10%CO2胁迫下，通过诱导抗氧化相关基因和酶的作用，抑制了活性氧的产生，从而减轻了鲜切梨的氧化损伤，保持了鲜切梨的贮藏品质。
Fresh-cut pear are popular for their nutritive, fresh and convenience. However, the oxidative stress caused by wounding aggravates quality loss. In the present study, the effects of 10 % CO2on reactive oxygen species (ROS) metabolism and antioxidant capacity in fresh-cut pear were investigated. The results showed that 10 % CO2effectively inhibited ROS accumulation during a 5-day storage, accompanied by reducing the content of hydrogen peroxide (H2O2) and superoxide anion radical (O2−). Furthermore, non-enzymatic antioxidant properties including free radical scavenging ability (DPPH), total antioxidant capacity (ABTS), and ferrous reducing ability (FRAP) in CO2-treated group were increased by 5.5 %, 23 % and 27 % respectively at the end of storage. Additionally, 10 % CO2treatment enhanced the activities and genes expression levels of catalase (CAT), glutathione peroxidase (GPX), ascorbate peroxidase (APX), glutathione reductase (GR), superoxide dismutase (SOD) and peroxidase (POD), while NADPH oxidase (NOX) were inhibited. The accumulation of ascorbic acid (AsA) and reduced glutathione (GSH) were also accelerated by CO2. This study indicated that the generation of ROS was inhibited with inducing effects of genes and enzymes related to antioxidant capacity in response to 10 % CO2, which eventually alleviated oxidative damage and conserved storage quality of fresh-cut pear.
登录
查看更多内容
CO2升高对采后草莓果实能量代谢和γ-氨基丁酸分流途径的影响
影响因子：
8.8
作者：
Li, Dong;Li, Li;Luo, Zisheng
通讯作者：
Luo, Zisheng
采后 ASM 浸渍和 DPI 预处理调节甜瓜 (Cucumis melo L.) 果实的活性氧代谢
影响因子：
7
作者：
Ge, Yonghong;Deng, Huiwen;Dong, Boyu
通讯作者：
Dong, Boyu
影响因子：
3.3
作者：
Khan, Muhammad Rafiullah;Suwanamornlert, Panitee;Chonhenchob, Vanee
通讯作者：
Chonhenchob, Vanee
影响因子：
4.3
作者：
Chumyam, Athiwat;Faiyue, Bualuang;Saengnil, Kobkiat
通讯作者：
Saengnil, Kobkiat
影响因子：
8.8
作者：
Ali, Sajid;Khan, Ahmad Sattar;Shahid, Muhammad
通讯作者：
Shahid, Muhammad