Stimulation of tyrosine-specific protein phosphorylation and phosphatidylinositol phosphorylation by orthovanadate in rat liver plasma membrane.
Stimulation of tyrosine-specific protein phosphorylation and phosphatidylinositol phosphorylation by orthovanadate in rat liver plasma membrane.
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原钒酸盐刺激大鼠肝质膜中酪氨酸特异性蛋白磷酸化和磷脂酰肌醇磷酸化。
DOI：
10.1016/0003-9861(89)90481-5
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发表时间：
1989
影响因子：
3.9
通讯作者：
Chan,TM
中科院分区：
文献类型：
--
作者：
Yang,DC;Brown,AB;Chan,TM
原钒酸可刺激Triton X-100溶解的大鼠肝细胞质膜上的[γ-32P]~(32)P掺入内源性三氯乙酸可沉淀的物质中，并加入(谷氨酸：酪氨酸)共聚物。用氯仿-甲醇-盐酸提取孵育混合物的结果表明，钒酸盐增加~(32)P的掺入主要是内源性磷脂。[32P]薄层层析鉴定磷脂酰肌醇4-磷酸(PtdIns-4-P)是钒酸刺激的主要磷酸化产物，它也导致内源膜蛋白中以P-Tyr为主的32P升高。钒酸对蛋白质酪氨酸和磷脂酰肌醇磷酸化的影响是相伴的，表现出相似的敏感性。二硫苏糖醇或NAD(P)H的存在可增强钒酸盐的上述作用，合成的抑制性酪氨酸激酶共聚物也可刺激或抑制磷脂酰肌醇的磷酸化。这些结果表明，氧自由基生成物钒酸刺激酪氨酸激酶-PtdIns激酶偶联系统，就像对许多生长因子和癌基因编码产物所描述的那样。
Orthovanadate stimulated the incorporation of32P from [γ-32P]ATP by Triton X-100-solubilized rat liver plasma membrane into endogenous, trichloroacetic acid-precipitable materials as well as added (Glu4:Tyr1) copolymers. Extraction of incubation mixture with chloroform-methanol-HCl revealed that the increase in32P incorporation by vanadate was predominantly into endogenous phospholipids. [32P]Phosphatidylinositol 4-phosphate (PtdIns-4-P) was identified by thin-layer chromatography as the major phosphorylated product of vanadate stimulation, which also resulted in elevated32P, predominantly in P-Tyr in endogenous membrane proteins. Vanadate effects on protein tyrosine and phosphatidylinositol phosphorylation were concomitant and exhibited similar sensitivity. These effects of vanadate were enhanced by the presence of either dithiothreitol or NAD(P)H. Phosphatidylinositol phosphorylation could also be stimulated by a substrate of and inhibited by a synthetic inhibitory copolymer of tyrosine kinase. These results suggest that vanadate, an oxygen radical producer, stimulates a tyrosine kinase-PtdIns kinase coupled system much like those described for a number of growth factors and oncogene encoded products.
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发育中的鸡胚上皮和间质的组织和精细结构
DOI：
--
发表时间：
1968
期刊：
影响因子：
--
作者：
E. Hay
通讯作者：
E. Hay
通过扫描显微镜观察上皮细胞和间充质细胞对基底层培养的反应。
影响因子：
3.7
作者：
J. Overton
通讯作者：
J. Overton
细胞在重组鼠尾胶原蛋白透明凝胶上的生长。
DOI：
--
发表时间：
1956
期刊：
Journal of the National Cancer Institute
影响因子：
--
作者：
R. L. Ehrmann;G. Gey
通讯作者：
G. Gey
影响因子：
4.2
作者：
P. Henkind;P. Prose
通讯作者：
P. Prose
影响因子：
7.8
作者：
Bard, J B;Hay, E D
通讯作者：
Hay, E D