Structural and mechanical properties of folded protein hydrogels with embedded microbubbles.
Structural and mechanical properties of folded protein hydrogels with embedded microbubbles.
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DOI：
10.1039/d2bm01918c
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发表时间：
2023-04-11
影响因子：
6.6
通讯作者：
Dougan L
中科院分区：
文献类型：
--
作者：
Brown CP;Hughes MDG;Mahmoudi N;Brockwell DJ;Coletta PL;Peyman S;Evans SD;Dougan L
球状折叠蛋白质是产生具有机械鲁棒性和固有生物功能性的生物材料的强大构建块。在这里，我们探索其作为先进的药物输送支架的潜力，通过嵌入微泡（MB）内的光活化，化学交联的牛血清白蛋白（BSA）蛋白质网络。使用圆二色性（CD），流变学，小角中子散射（SANS）和显微镜的组合，我们确定这种新型的多复合系统的纳米尺度和介观尺度的结构和力学。光学和共聚焦显微镜证实了蛋白质水凝胶中MB的存在、它们的扩散减少以及使用超声进行的有效破裂，这是药物爆发释放的需要。CD证实，包含MB不会影响交联蛋白质网络内折叠蛋白质的比例。流变学表征表明，在MB的存在下，BSA水凝胶的力学性能降低。此外，SANS揭示了将MB嵌入蛋白质水凝胶网络中导致更小数量的尺寸更大的簇（簇数量减少约16.6%，簇尺寸增加17.4%）。两者合计，我们表明，MB可以成功地嵌入折叠的蛋白质网络，并在应用超声时破裂。在纳米级和中尺度的蛋白质支架中嵌入MB的影响的基本见解是重要的，在未来的平台的发展，有针对性的和控制的药物输送应用。球状折叠蛋白质是产生具有机械鲁棒性和固有生物功能性的生物材料的强大构建块。
Globular folded proteins are powerful building blocks to create biomaterials with mechanical robustness and inherent biological functionality. Here we explore their potential as advanced drug delivery scaffolds, by embedding microbubbles (MBs) within a photo-activated, chemically cross-linked bovine serum albumin (BSA) protein network. Using a combination of circular dichroism (CD), rheology, small angle neutron scattering (SANS) and microscopy we determine the nanoscale and mesoscale structure and mechanics of this novel multi-composite system. Optical and confocal microscopy confirms the presence of MBs within the protein hydrogel, their reduced diffusion and their effective rupture using ultrasound, a requirement for burst drug release. CD confirms that the inclusion of MBs does not impact the proportion of folded proteins within the cross-linked protein network. Rheological characterisation demonstrates that the mechanics of the BSA hydrogels is reduced in the presence of MBs. Furthermore, SANS reveals that embedding MBs in the protein hydrogel network results in a smaller number of clusters that are larger in size (∼16.6% reduction in number of clusters, 17.4% increase in cluster size). Taken together, we show that MBs can be successfully embedded within a folded protein network and ruptured upon application of ultrasound. The fundamental insight into the impact of embedded MBs in protein scaffolds at the nanoscale and mesoscale is important in the development of future platforms for targeted and controlled drug delivery applications. Globular folded proteins are powerful building blocks to create biomaterials with mechanical robustness and inherent biological functionality.
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影响因子：
16.6
作者：
Bian Q;Fu L;Li H
通讯作者：
Li H
DOI：
10.1073/pnas.96.11.6020
发表时间：
1999-05-25
影响因子：
11.1
作者：
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通讯作者：
Kodadek, T
影响因子：
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作者：
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通讯作者：
Lentacker, Ine
DOI：
10.1016/j.nano.2021.102401
发表时间：
2021-07-22
影响因子：
5.4
作者：
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通讯作者：
Coletta, P. Louise
影响因子：
3.9
作者：
Fang, Jie;Li, Hongbin
通讯作者：
Li, Hongbin