Engineering shape memory and morphing protein hydrogels based on protein unfolding and folding.
Engineering shape memory and morphing protein hydrogels based on protein unfolding and folding.
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DOI：
10.1038/s41467-021-27744-0
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发表时间：
2022-01-10
影响因子：
16.6
通讯作者：
Li H
中科院分区：
文献类型：
--
作者：
Bian Q;Fu L;Li H
工程形状记忆/变形材料在聚合物基系统中取得了相当大的进展，具有广泛的潜在应用。然而，工程蛋白质为基础的形状记忆/变形材料仍然具有挑战性和探索不足。在这里，我们报告了一个双层蛋白质为基础的形状记忆/变形水凝胶的蛋白质折叠-展开机制的基础上设计。我们制造的蛋白质双层结构使用两个串联模块弹性蛋白（GB 1）8和（FL）8。这两个蛋白质层显示不同的变性剂依赖性的溶胀曲线和杨氏模量。由于这种蛋白质展开-折叠诱导的溶胀变化，双层水凝胶显示出高度可调和可逆的双向弯曲变形，这取决于变性剂浓度和层几何形状。基于这些可编程和可逆的弯曲行为，我们进一步利用蛋白质双层结构作为铰链，实现了图案化水凝胶的一维到二维和二维到三维的折叠转换。本文的工作将为新型形变材料的设计和制备提供新的启示。基于聚合物的形状记忆和变形材料的研究取得了很大的进展，但基于蛋白质的形状记忆和变形材料的研究仍然很少。在这里，作者报告了基于蛋白质的形状记忆和变形水凝胶的工程设计，使用蛋白质折叠-展开作为触发蛋白质双层结构中形状变形的一般机制。
Engineering shape memory/morphing materials have achieved considerable progress in polymer-based systems with broad potential applications. However, engineering protein-based shape memory/morphing materials remains challenging and under-explored. Here we report the design of a bilayer protein-based shape memory/morphing hydrogel based on protein folding-unfolding mechanism. We fabricate the protein-bilayer structure using two tandem modular elastomeric proteins (GB1)8 and (FL)8. Both protein layers display distinct denaturant-dependent swelling profiles and Young’s moduli. Due to such protein unfolding-folding induced changes in swelling, the bilayer hydrogels display highly tunable and reversible bidirectional bending deformation depending upon the denaturant concentration and layer geometry. Based on these programmable and reversible bending behaviors, we further utilize the protein-bilayer structure as hinge to realize one-dimensional to two-dimensional and two-dimensional to three-dimensional folding transformations of patterned hydrogels. The present work will offer new inspirations for the design and fabrication of novel shape morphing materials. Engineering shape memory and morphing materials achieved considerable progress in polymer-based systems, but protein-based shape memory and morphing materials remain less investigated. Here, the authors report the engineering of protein-based shape memory and morphing hydrogels using protein folding-unfolding as a general mechanism to trigger shape morphing in protein-bilayer structures.
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影响因子：
10
作者：
An, Young-Hyeon;Yu, Seung Jung;Hwang, Nathaniel S.
通讯作者：
Hwang, Nathaniel S.
影响因子：
3.9
作者：
Fang, Jie;Li, Hongbin
通讯作者：
Li, Hongbin
DOI：
10.1073/pnas.96.11.6020
发表时间：
1999-05-25
影响因子：
11.1
作者：
Fancy, DA;Kodadek, T
通讯作者：
Kodadek, T
影响因子：
13.6
作者：
Khoury, Luai R.;Slawinski, Marina;Popa, Ionel
通讯作者：
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影响因子：
16.6
作者：
Fang, L.;Jia, Y.;Kwok, W. K.
通讯作者：
Kwok, W. K.