Hexuronic acid stereochemistry determination in chondroitin sulfate glycosaminoglycan oligosaccharides by electron detachment dissociation.
Hexuronic acid stereochemistry determination in chondroitin sulfate glycosaminoglycan oligosaccharides by electron detachment dissociation.
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DOI：
10.1007/s13361-012-0428-5
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发表时间：
2012-09
影响因子：
3.2
通讯作者：
Amster, I. Jonathan
中科院分区：
文献类型：
--
作者：
Leach, Franklin E., III;Ly, Mellisa;Laremore, Tatiana N.;Wolff, Jeremy J.;Perlow, Jacob;Linhardt, Robert J.;Amster, I. Jonathan
关键词：
电子分离解离（EDD）以前提供了立体特异性产物离子，允许分配硫酸乙酰肝素糖胺聚糖（GAG）中的酸性C-5立体化学，但将相同的方法应用于硫酸软骨素糖型类中的差向异构体对不能提供相同的结果。通过电子解离（EDD）、电子诱导解离（EID）和负电子转移解离（NETD）分别激活糖胺聚糖前体离子，对硫酸软骨素（CS）差向异构体的立体化学进行了归属，并研究了CS糖型EDD过程中产物离子的形成机制。这种方法允许分配的电子激发产品形成的EID和脱离产品的自由基途径在NETD，这两者同时发生在EDD。糖醛酸立体化学的电子分离光谱产生的强度差异时，分配糖苷和交叉环裂解进行比较。双去质子化的0、2X 3和Y3离子的强度的变化已被证明是二元混合物的CID期间CS-A/DS组成的指示。这些离子可以提供洞察EDD中的二元混合物的糖醛酸组合物，但相对丰度，虽然可重复的，是低的相比，在离子阱上获得的CID光谱。主成分分析（PCA）的应用程序提出了一种多变量的方法来确定糖醛酸立体化学光谱的这些糖胺聚糖利用电子分离产生的可重复的峰分布。
Electron detachment dissociation (EDD) has previously provided stereo-specific product ions that allow for the assignment of the acidic C-5stereochemistry in heparan sulfate glycosaminoglycans (GAGs), but application of the same methodology to an epimer pair in the chondroitin sulfate glycoform class does not provide the same result. A series of experiments have been conducted in which glycosaminoglycan precursor ions are independently activated by electron detachment dissociation (EDD), electron induced dissociation (EID), and negative electron transfer dissociation (NETD) to assign the stereochemistry in chondroitin sulfate (CS) epimers and investigate the mechanisms for product ion formation during EDD in CS glycoforms. This approach allows for the assignment of electronic excitation products formed by EID and detachment products to radical pathways in NETD, both of which occur simultaneously during EDD. The uronic acid stereochemistry in electron detachment spectra produces intensity differences when assigned glycosidic and cross-ring cleavages are compared. The variations in the intensities of the doubly deprotonated 0,2X3 and Y3 ions have been shown to be indicative of CS-A/DS composition during the CID of binary mixtures. These ions can provide insight into the uronic acid composition of binary mixtures in EDD, but the relative abundances, although reproducible, are low compared with those in a CID spectrum acquired on an ion trap. The application of principal component analysis (PCA) presents a multivariate approach to determining the uronic acid stereochemistry spectra of these GAGs by taking advantage of the reproducible peak distributions produced by electron detachment.
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影响因子：
7.4
作者：
Desaire, H;Sirich, TL;Leary, JA
通讯作者：
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影响因子：
2.9
作者：
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DOI：
10.1016/1044-0305(92)80002-3
发表时间：
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影响因子：
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通讯作者：
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DOI：
10.1007/s13361-011-0174-0
发表时间：
2011-09
影响因子：
3.2
作者：
Huang, Rongrong;Pomin, Vitor H.;Sharp, Joshua S.
通讯作者：
Sharp, Joshua S.