Characterization of oral and gut microbiome and plasma metabolomics in COVID-19 patients after 1-year follow-up.
Characterization of oral and gut microbiome and plasma metabolomics in COVID-19 patients after 1-year follow-up.
复制标题
DOI：
10.1186/s40779-022-00387-y
复制
发表时间：
2022-06-17
影响因子：
21.1
通讯作者：
中科院分区：
文献类型：
--
作者：
由于 2019 年冠状病毒病 (COVID-19) 的爆发和迅速传播，迄今为止，全球已有超过 1.6 亿患者成为康复者。 COVID-19 患者的肠道和口腔微生物组以及代谢组学发生了显着变化。但恢复1年后其特征是否恢复正常尚不清楚。我们招募了 35 名确诊患者，以及 160 名健康对照者，在出院时和一年后提供样本。前瞻性收集样本共497份，其中舌苔样本219份、粪便样本129份、血浆样本149份。对舌苔和粪便样本进行 16S rRNA 测序，对血浆样本进行非靶向代谢组学测试。 1年恢复期患者的口腔和肠道微生物组和代谢组学特征在很大程度上恢复，但并未完全恢复正常。在恢复过程中，微生物多样性逐渐增加。产丁酸微生物和双歧杆菌逐渐增多，而产脂多糖微生物逐渐减少。此外，与COVID-19炎症因子风暴密切相关的1-磷酸鞘氨醇在恢复过程中显着升高。此外，根据患者出院时的微生物组和代谢物建立的预测模型在预测一年后的中和抗体水平方面达到了很高的效果。这项研究首次对 COVID-19 恢复期一年的患者的口腔和肠道微生物组和代谢组学进行了表征。确定了康复过程中的关键微生物组和代谢物，为加速康复提供了新的治疗思路。基于微生物组和代谢组学的预测模型为预测恢复情况提供了新的见解，这有利于受影响的个人和医疗保健。在线版本包含可在 10.1186/s40779-022-00387-y 获取的补充材料。
Due to the outbreak and rapid spread of coronavirus disease 2019 (COVID-19), more than 160 million patients have become convalescents worldwide to date. Significant alterations have occurred in the gut and oral microbiome and metabonomics of patients with COVID-19. However, it is unknown whether their characteristics return to normal after the 1-year recovery. We recruited 35 confirmed patients to provide specimens at discharge and one year later, as well as 160 healthy controls. A total of 497 samples were prospectively collected, including 219 tongue-coating, 129 stool and 149 plasma samples. Tongue-coating and stool samples were subjected to 16S rRNA sequencing, and plasma samples were subjected to untargeted metabolomics testing. The oral and gut microbiome and metabolomics characteristics of the 1-year convalescents were restored to a large extent but did not completely return to normal. In the recovery process, the microbial diversity gradually increased. Butyric acid-producing microbes and Bifidobacterium gradually increased, whereas lipopolysaccharide-producing microbes gradually decreased. In addition, sphingosine-1-phosphate, which is closely related to the inflammatory factor storm of COVID-19, increased significantly during the recovery process. Moreover, the predictive models established based on the microbiome and metabolites of patients at the time of discharge reached high efficacy in predicting their neutralizing antibody levels one year later. This study is the first to characterize the oral and gut microbiome and metabonomics in 1-year convalescents of COVID-19. The key microbiome and metabolites in the process of recovery were identified, and provided new treatment ideas for accelerating recovery. And the predictive models based on the microbiome and metabolomics afford new insights for predicting the recovery situation which benefited affected individuals and healthcare. The online version contains supplementary material available at 10.1186/s40779-022-00387-y.
登录
查看更多内容
影响因子：
24.5
作者：
Ren Z;Wang H;Cui G;Lu H;Wang L;Luo H;Chen X;Ren H;Sun R;Liu W;Liu X;Liu C;Li A;Wang X;Rao B;Yuan C;Zhang H;Sun J;Chen X;Li B;Hu C;Wu Z;Yu Z;Kan Q;Li L
通讯作者：
Li L
影响因子：
25.7
作者：
Beyoglu, Diren;Idle, Jeffrey R.
通讯作者：
Idle, Jeffrey R.
影响因子：
4.8
作者：
Haenel, Petra;Andreani, Paul;Graeler, Markus H.
通讯作者：
Graeler, Markus H.
影响因子：
168.9
作者：
Huang, Chaolin;Wang, Yeming;Cao, Bin
通讯作者：
Cao, Bin
影响因子：
82.9
作者：
Blomberg B;Mohn KG;Brokstad KA;Zhou F;Linchausen DW;Hansen BA;Lartey S;Onyango TB;Kuwelker K;Sævik M;Bartsch H;Tøndel C;Kittang BR;Bergen COVID-19 Research Group;Cox RJ;Langeland N
通讯作者：
Langeland N