Human pulmonary macrophage-derived mucus secretagogue-猫眼课题宝

人肺巨噬细胞衍生的粘液分泌素

DOI：

发表时间：

1984

期刊：

Journal of Experimental Medicine

影响因子：

15.3

通讯作者：

中科院分区：

医学1区

文献类型：

作者：

Z. Marom;J. Shelhamer;M. Kaliner

从手术切除的人肺组织中获得的人肺巨噬细胞（PM）在暴露于活化的酵母聚糖后释放一种因子，该因子导致培养的人气道释放增加量的放射性标记的粘液糖蛋白。该因子在酵母聚糖暴露4-8小时后释放最大，并引起糖蛋白释放的剂量相关性增加;它被称为巨噬细胞来源的粘液促分泌素（MMS）。MMS释放产生的剂量依赖性的方式，由激活，但不是由非激活酵母聚糖。酵母多糖的激活是C3依赖的，C3 b包被的琼脂糖凝胶也是一种有效的刺激剂。数据表明PM的细胞表面活化是引起MMS释放的充分刺激，并且C3依赖性活化以及Fc受体活化都是有效的。MMS的合成对放线菌酮敏感，细胞内检测不到活性MMS。为了确定MMS是否可能是花生四烯酸的氧化衍生物之一，在活化前将PM与环氧合酶和脂氧合酶抑制剂一起孵育。这些演习没有影响MMS的生成。然后将富含MMS的上清液提取到有机溶剂中或暴露于亲脂性树脂;在这两种情况下，MMS保留在水相中。因此，MMS不是花生四烯酸的衍生物。MMS的顺序分馏超膜和凝胶过滤，然后等电聚焦和凝胶过滤表明，MMS是一个小的（约2000道尔顿），酸性（pI，5.15）分子。因此，人PM的表面活化导致小的酸性分子的合成和释放，其导致气道粘液腺分泌增加量的粘液糖蛋白。

Human pulmonary macrophages (PM) obtained from surgically removed human lung tissue released a factor after exposure to activated zymosan that caused cultured human airways to release increased amounts of radiolabeled mucous glycoproteins. The factor was released maximally after 4-8 h of zymosan exposure and caused a dose-related increase in glycoprotein release; it was termed macrophage-derived mucus secretagogue (MMS). MMS release was produced in a dose-dependent fashion by activated but not by nonactivated zymosan. The activation of zymosan was C3 dependent, and C3b-coated Sepharose was also an effective stimulant. The data suggested that cell surface activation of the PM was a sufficient stimulus to cause MMS release and that both C3- dependent activation as well as Fc receptor activation were effective. The synthesis of MMS was sensitive to cycloheximide, and no active MMS was detectable intracellularly. To determine if MMS might be one of the oxidative derivatives of arachidonic acid, PM were incubated with cyclooxygenase and lipoxygenase inhibitors before activation. These maneuvers did not influence MMS generation. MMS-rich supernatants were then extracted into organic solvents or exposed to lipophilic resin; in both cases, MMS remained in the aqueous phase. Thus, MMS is not a derivative of arachidonic acid. Sequential fractionation of MMS on ultramembrane and gel filtration followed by isoelectric focusing and gel filtration indicated that MMS is a small (approximately 2000 daltons), acidic (pI, 5.15) molecule. Therefore, surface activation of human PM results in the synthesis and release of a small acidic molecule that causes airway mucous glands to secrete increased quantities of mucous glycoproteins.

Mouse peritoneal macrophages release leukotriene C in response to a phagocytic stimulus.