Transient receptor potential is essential for high temperature tolerance in invasive Bemisia tabaci Middle East Asia minor 1 cryptic species.
Transient receptor potential is essential for high temperature tolerance in invasive Bemisia tabaci Middle East Asia minor 1 cryptic species.
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DOI：
10.1371/journal.pone.0108428
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Wan FH
中科院分区：
文献类型：
--
作者：
Lü ZC;Li Q;Liu WX;Wan FH
温度是影响生物种群动态和扩散分布的重要因子。外来物种能够在各种温度环境中成功入侵和定殖，重要原因之一是外来物种对温度胁迫具有较强的抵抗力。近年来，研究者们开始关注温度感受机制，以确定温度适应的感受和调节机制。瞬时感受器电位（TRP）是生物体温度感知系统的关键组成部分之一。TRP在感知温度中起着避高温、避低温、选择最佳温度等重要作用。为研究TRP基因的高温感受性和耐热性，采用3′和5′末端快速扩增技术克隆了烟粉虱MEAM1（Middle East Asia Minor 1）TRP基因的全长cDNA序列，分析了其在不同温度条件下的mRNA表达谱，并鉴定了其耐热性功能。这是首次对侵袭性B的TRP基因进行研究。烟粉虱MEAM1（MEAM1 BtTRP）。MEAM 1 BtTRP的cDNA全长为3871 bp，开放阅读框为3501 bp，编码1166个氨基酸。此外，在35°C下，BtTRP mRNA表达水平显著增加。与对照组相比，处理B.在高温胁迫条件下，取食dsRNA BtTRP后，烟粉虱MEAM 1成虫的生长量显著下降。总的来说，这些结果表明MEAM 1 BtTRP是感知高温的关键元件，并且在B中起重要作用。烟粉虱MEAM1耐热性我们的数据提高了我们对B温度感觉机制的理解。烟粉虱MEAM 1的分子生物学特性，为进一步研究B的热生物学提供了理论依据。全球气候变化背景下的烟粉虱MEAM1。
Temperature is an important factor in affecting population dynamics and diffusion distribution of organisms. Alien species can successfully invade and colonize to various temperature environments, and one of important reasons is that alien species have a strong resistance to stress temperature. Recently, researchers have focused on the mechanisms of temperature sensing to determine the sensing and regulation mechanisms of temperature adaptation. The transient receptor potential (TRP) is one of the key components of an organism’s temperature perception system. TRP plays important roles in perceiving temperature, such as avoiding high temperature, low temperature and choosing the optimum temperature. To assess high temperature sensation and the heat resistance role of the TRP gene, we used 3′ and 5′ rapid-amplification of cDNA ends to isolate the full-length cDNA sequence of the TRP gene from Bemisia tabaci (Gennadius) MEAM1 (Middle East Asia Minor 1), examined the mRNA expression profile under various temperature conditions, and identified the heat tolerance function. This is the first study to characterize the TRP gene of invasive B. tabaci MEAM1 (MEAM1 BtTRP). The full-length cDNA of MEAM1 BtTRP was 3871 bp, and the open reading frames of BtTRP was 3501 bp, encoding 1166 amino acids. Additionally, the BtTRP mRNA expression level was significantly increased at 35°C. Furthermore, compared with control treatments, the survival rate of B. tabaci MEAM1 adults was significantly decreased under high temperature stress conditions after feeding with dsRNA BtTRP. Collectively, these results showed that MEAM1 BtTRP is a key element in sensing high temperature and plays an essential role in B. tabaci MEAM1 heat tolerance ability. Our data improved our understanding of the mechanism of temperature sensation in B. tabaci MEAM1 at the molecular level and could contribute to the understanding of the thermal biology of B. tabaci MEAM1 within the context of global climate change.
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DOI：
10.1002/ps.2780420211
发表时间：
1994-10-01
期刊：
PESTICIDE SCIENCE
影响因子：
--
作者：
CHEEK, S;MACDONALD, O
通讯作者：
MACDONALD, O
影响因子：
2.8
作者：
Oliveira, MRV;Henneberry, TJ;Anderson, P
通讯作者：
Anderson, P
影响因子：
3.8
作者：
Ghanim, Murad;Kontsedalov, Svetlana;Czosnek, Henryk
通讯作者：
Czosnek, Henryk
影响因子：
3.7
作者：
Neely GG;Keene AC;Duchek P;Chang EC;Wang QP;Aksoy YA;Rosenzweig M;Costigan M;Woolf CJ;Garrity PA;Penninger JM
通讯作者：
Penninger JM
影响因子：
6.1
作者：
Fowler, Melissa A.;Montell, Craig
通讯作者：
Montell, Craig