Detection of hypoxia in human brain tumor xenografts using a modified comet assay.
Detection of hypoxia in human brain tumor xenografts using a modified comet assay.
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使用改良的彗星测定检测人脑肿瘤异种移植物中的缺氧。
DOI：
10.1016/s1476-5586(03)80022-2
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Deen,DennisF
中科院分区：
文献类型：
--
作者：
Wang,Jingli;Klem,Jack;Wyrick,JanB;Ozawa,Tomoko;Cunningham,Erin;Golinveaux,Jay;Allen,MaxJ;Lamborn,KathleenR;Deen,DennisF
我们使用标准的彗星实验成功地产生了缺氧和缺氧条件下的玻璃剂量-反应曲线。然后，我们用3GyX射线和9GyX射线照射的混合细胞来模拟肿瘤中的两个亚群，但使用标准彗星分析准确检测和量化亚群的努力没有成功。因此，我们研究了一种改进的彗星试验，以确定它是否可以用于在我们的模型系统中测量缺氧。U251 MG细胞作为裸鼠皮下肿瘤生长，U251 MG和U87 MG细胞作为脑内(I.C.)细胞生长。无瘤大鼠的肿瘤。动物注射RSU 1069，照射后安乐死。切除肿瘤和正常脑组织，并使用改进的彗星试验对细胞进行分析。肿瘤和对侧正常脑之间彗星尾矩分布的差异，使用每个分布中第25或50个百分位数的尾矩，作为衡量肿瘤缺氧程度的指标。对于U251 MG肿瘤，肿瘤大小与缺氧程度呈正相关，而来自U87 MG I.C.的初步数据。肿瘤表现为低氧，肿瘤大小与低氧无明显关系。
We used the standard comet assay successfully to generatein vitrodose-response curves under oxic and hypoxic conditions. We then made mixtures of cells that had been irradiated with 3 and 9 Gy of X-rays to simulate two subpopulations in a tumor, but efforts to accurately detect and quantify the subpopulations using the standard comet assay were unsuccessful. Therefore, we investigated a modified comet assay to determine whether it could be used for measuring hypoxia in our model systems. U251 MG cells were grown as subcutaneous tumors in athymic mice; U251 MG and U87 MG cells were grown as intracerebral (i.c.) tumors in athymic rats. Animals were injected with RSU 1069, irradiated, and euthanized. Tumors and normal brains were removed, and the cells were analyzed using a modified comet assay. Differences in comet tail moment distributions between tumor and contralateral normal brain, using tail moments at either the 25th or 50th percentile in each distribution, were taken as measures of the degree of tumor hypoxia. For U251 MG tumors, there was a positive relationship between tumor size and the degree of hypoxia, whereas preliminary data from U87 MG i.c. tumors showed less hypoxia and no apparent relationship between tumor size and hypoxia.
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影响因子：
11.2
作者：
Janice M. Y. Brown;A. Giaccia
通讯作者：
Janice M. Y. Brown;A. Giaccia
影响因子：
8.8
作者：
Olive, P L
通讯作者：
Olive, P L
DOI：
10.1093/jnci/84.9.707
发表时间：
1992-05-06
期刊：
JOURNAL OF THE NATIONAL CANCER INSTITUTE
影响因子：
--
作者：
OLIVE, PL;DURAND, RE
通讯作者：
DURAND, RE
DOI：
10.1016/s0360-3016(98)00332-0
发表时间：
1998-11-01
影响因子：
7
作者：
Horsman, MR
通讯作者：
Horsman, MR
影响因子：
11.2
作者：
Peggy L. Olive;Ralph E. Durand;J. L. Riche;Ivo A. Olivotto;Stewart M. Jackson
通讯作者：
Peggy L. Olive;Ralph E. Durand;J. L. Riche;Ivo A. Olivotto;Stewart M. Jackson