OsWRKY93 Dually Functions Between Leaf Senescence and in Response to Biotic Stress in Rice.
OsWRKY93 Dually Functions Between Leaf Senescence and in Response to Biotic Stress in Rice.
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OsWRKY93 在水稻叶片衰老和响应生物胁迫之间发挥双重作用
DOI：
10.3389/fpls.2021.643011
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发表时间：
2021
影响因子：
5.6
通讯作者：
Miao Y
中科院分区：
文献类型：
--
作者：
Li Y;Liao S;Mei P;Pan Y;Zhang Y;Zheng X;Xie Y;Miao Y
自然衰老与病原体攻击引起的细胞死亡之间的交互作用是一个有趣的话题，然而，其作用机制仍然是开放的。本研究利用RNA-seq数据集，通过叶片衰老程序筛选得到33个OsWRKY基因，并利用RT-qPCR技术证实其中11个OsWRKY基因在旗叶衰老过程中表达水平发生了显著变化。其中，OsWRKY 2、OsWRKY 14、OsWRKY 26、OsWRKY 69和OsWRKY 93成员在响应稻瘟菌感染时表现出转录水平的短期改变。开发并证实了5个基因的CRISPR/Cas9编辑突变体，并且对M.在CRISPR 0 sWRKY 93编辑的株系中观察到了对M. OsWRKY 93基因表达增强的植株与对照植株相比，表现出明显的外源基因感染，而其他4个基因的表达增强对OsWRKY 93基因表达的影响不明显。有趣的是，与对照相比，在flg 22处理后，0 sWRKY 93增强的植株中ROS积累也增加，表明0 sWRKY 93参与PAMP触发的水稻免疫应答。这表明OsWRKY 93既参与旗叶衰老，又参与对真菌攻击的响应。
Cross talking between natural senescence and cell death in response to pathogen attack is an interesting topic; however, its action mechanism is kept open. In this study, 33 OsWRKY genes were obtained by screening with leaf aging procedure through RNA-seq dataset, and 11 of them were confirmed a significant altered expression level in the flag leaves during aging by using the reverse transcript quantitative PCR (RT-qPCR). Among them, the OsWRKY2, OsWRKY14, OsWRKY26, OsWRKY69, and OsWRKY93 members exhibited short-term alteration in transcriptional levels in response to Magnaporthe grisea infection. The CRISPR/Cas9-edited mutants of five genes were developed and confirmed, and a significant sensitivity to M. oryzae infection was observed in CRISPR OsWRKY93-edited lines; on the other hand, a significant resistance to M. oryzae infection was shown in the enhanced expression OsWRKY93 plants compared to mock plants; however, enhanced expression of other four genes have no significant affection. Interestingly, ROS accumulation was also increased in OsWRKY93 enhanced plants after flg22 treatment, compared with the controls, suggesting that OsWRKY93 is involved in PAMP-triggered immune response in rice. It indicated that OsWRKY93 was involved in both flag leaf senescence and in response to fungi attack.
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