Comparative Transcriptome Profiling of Two Tomato Genotypes in Response to Potassium-Deficiency Stress.
Comparative Transcriptome Profiling of Two Tomato Genotypes in Response to Potassium-Deficiency Stress.
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DOI：
10.3390/ijms19082402
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发表时间：
2018-08-14
影响因子：
5.6
通讯作者：
Jiang J
中科院分区：
文献类型：
--
作者：
Zhao X;Liu Y;Liu X;Jiang J
番茄是一种需要充足钾（K）供应以获得最佳产量和品质的作物。缺钾胁迫降低番茄产量和品质。为了进一步研究番茄对低钾胁迫的响应机制，筛选番茄耐低钾基因，以耐低钾基因型JZ 34和低钾敏感基因型JZ 18为材料，采用BGISEQ-500进行了RNA测序。在缺钾处理后12 h和24 h，JZ 18和JZ 34共鉴定出1936个差异表达基因（DEG）。根据基因本体论（GO）和京都基因与基因组百科全书（KEGG）途径分析，显著改变的DEG主要包括转录因子、转运蛋白、激酶、氧化应激蛋白以及激素信号和糖代谢相关基因。实验结果证实了低K+信号途径中响应基因的诱导表达。最大的DEG组包括多达110个氧化应激相关基因。总共有19个乙烯反应因子（ERF）表现出JZ 18和JZ 34之间的差异表达，响应K+缺乏。此外，我们还通过定量RT-PCR（qRT-PCR）技术，确定了20个与缺钾胁迫密切相关的DEG，其中一些DEG影响了番茄根系形态，这些DEG可作为分子靶标进一步研究，探索新的途径，获得番茄更有效的钾吸收效率。提出了一个假说，涉及可能的植物激素信号线索和活性氧（ROS）之间的串扰导致根生长在JZ 34。这些结果为番茄对低K+胁迫响应的分子机制提供了全面的基础。
Tomato is a crop that requires a sufficient supply of potassium (K) for optimal productivity and quality. K+-deficiency stress decreases tomato yield and quality. To further delve into the mechanism of the response to K+-deficiency and to screen out low-K+ tolerant genes in tomatoes, BGISEQ-500-based RNA sequencing was performed using two tomato genotypes (low-K+ tolerant JZ34 and low-K+ sensitive JZ18). We identified 1936 differentially expressed genes (DEGs) in JZ18 and JZ34 at 12 and 24 h after K+-deficiency treatment. According to the Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analyses, the DEGs that changed significantly primarily included transcription factors, transporters, kinases, oxidative stress proteins, and hormone signaling-and glycometabolism-related genes. The experimental results confirmed the induced expression of the responsive genes in the low-K+ signaling pathway. The largest group of DEGs comprised up to 110 oxidative stress-related genes. In total, 19 ethylene response factors (ERFs) demonstrated differential expression between JZ18 and JZ34 in response to K+-deficiency. Furthermore, we confirmed 20 DEGs closely related to K+-deficiency stress by quantitative RT-PCR (qRT-PCR), some of which affected the root configuration, these DEGs could be further studied for use as molecular targets to explore novel approaches, and to acquire more effective K acquisition efficiencies for tomatoes. A hypothesis involving possible cross-talk between phytohormone signaling cues and reactive oxygen species (ROS) leading to root growth in JZ34 is proposed. The results provide a comprehensive foundation for the molecular mechanisms involved in the response of tomatoes to low K+ stress.
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影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
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通讯作者：
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作者：
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通讯作者：
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影响因子：
7.4
作者：
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