Cross talk between endothelial and red blood cell glycocalyces via near-field flow.
Cross talk between endothelial and red blood cell glycocalyces via near-field flow.
复制标题
DOI：
10.1016/j.bpj.2021.06.002
复制
发表时间：
2021-08-03
影响因子：
3.4
通讯作者：
Luo KH
中科院分区：
文献类型：
--
作者：
Jiang XZ;Goligorsky MS;Luo KH
血管内皮细胞和循环红细胞（RBC）表面都覆盖着一层浓密的糖萼。这些糖萼层之间的相互作用很难测量，也不充分了解。本研究旨在通过数学建模和数值模拟来研究和确定红细胞糖萼与内皮细胞之间可能的相互作用。采用耗散粒子动力学（DPD）模拟研究了内皮糖萼（EG）对不同环境条件的响应。建立了包括EG和血流的两室模型和包括EG、红细胞糖萼和血流的三室模型。双室分析表明，相对较快的流动主要与EG的弯曲运动有关，而相对缓慢的流动主要是振荡运动。结果表明，循环红细胞引起EG的非接触变形。它的变形取决于链的布局、链的长度、弯曲刚度、红细胞到eg的距离和红细胞的速度。具体来说，更短的EG链或红细胞到EG的距离导致EG的相对偏转更大。当EG链稀薄或红细胞移动更快时，EG的变形会增强。弯曲刚度保持EG的拉伸构象。此外，紧凑的EG链布局和脱落的EG链干扰了邻近的流场，导致流速分布紊乱。相反，EG链在红细胞表面的运动对红细胞的驱动作用很小。据我们所知，在三室系统中首次使用DPD方法来探索EG和红细胞糖萼之间的串扰。研究表明，红细胞通过近场流驱动脑电变形，而脑电对红细胞的边际推进作用被观察到。据我们所知，这些新的发现提供了一个新的角度来理解糖萼在机械传导和微血管通透性中的作用，以及它们在与EG降解相关的理想病理生理条件下的扰动。
Vascular endothelial cells and circulating red blood cell (RBC) surfaces are both covered by a layer of bushy glycocalyx. The interplay between these glycocalyx layers is hardly measurable and insufficiently understood. This study aims to investigate and qualify the possible interactions between the glycocalyces of RBCs and endothelial cells using mathematical modeling and numerical simulation. Dissipative particle dynamics (DPD) simulations are conducted to investigate the response of the endothelial glycocalyx (EG) to varying ambient conditions. A two-compartment model including EG and flow and a three-compartment model comprising EG, RBC glycocalyx, and flow are established. The two-compartment analysis shows that a relatively fast flow is associated with a predominantly bending motion of the EG, whereas oscillatory motions are predominant in a relatively slow flow. Results show that circulating RBCs cause the contactless deformation of EG. Its deformation is dependent on the chain layout, chain length, bending stiffness, RBC-to-EG distance, and RBC velocities. Specifically, shorter EG chains or RBC-to-EG distance leads to greater relative deflections of EG. Deformation of EG is enhanced when the EG chains are rarefied or RBCs move faster. The bending stiffness maintains stretching conformation of EG. Moreover, a compact EG chain layout and shedding EG chains disturb the neighboring flow field, causing disordered flow velocity distributions. In contrast, the movement of EG chains on RBC surfaces exerts a marginal driving force on RBCs. The DPD method is used for the first time, to our knowledge, in the three-compartment system to explore the cross talk between EG and RBC glycocalyx. This study suggests that RBCs drive the EG deformation via the near-field flow, whereas marginal propulsion of RBCs by the EG is observed. These new, to our knowledge, findings provide a new angle to understand the roles of glycocalyx in mechanotransduction and microvascular permeability and their perturbations under idealized pathophysiologic conditions associated with EG degradation.
登录
查看更多内容
影响因子：
4.6
作者：
Kabedev A;Lobaskin V
通讯作者：
Lobaskin V
影响因子：
4.6
作者：
Jiang, XiZhuo;Feng, Muye;Luo, Kai H.
通讯作者：
Luo, Kai H.
影响因子：
7.4
作者：
Masola V;Zaza G;Onisto M;Lupo A;Gambaro G
通讯作者：
Gambaro G
DOI：
10.1152/ajpheart.00467.2002
发表时间：
2003-04-01
影响因子：
4.8
作者：
Fu, BMM;Chen, B;Chen, WH
通讯作者：
Chen, WH
影响因子：
6.3
作者：
Jiang, Xi Zhuo;Luo, Kai H.;Ventikos, Yiannis
通讯作者：
Ventikos, Yiannis