Molecular cloning of a genetic determinant for Congo red binding ability which is essential for the virulence of Shigella flexneri
Molecular cloning of a genetic determinant for Congo red binding ability which is essential for the virulence of Shigella flexneri
复制标题
刚果红结合能力遗传决定因子的分子克隆，这对于福氏志贺氏菌的毒力至关重要
作者：
T. Sakai;C. Sasakawa;S. Makino;K. Kamata;M. Yoshikawa
通过选择结合刚果红的能力（Pcr+表型），将来自完全毒力福氏志贺菌2a菌株YSH 6000中的230-kb（140兆道尔顿）质粒的约1.0 kb的DNA序列克隆到大肠杆菌K-12中。将其定位在质粒上的SalI限制性片段F内。这个克隆人把一个S.弗氏菌菌株，该菌株由于质粒中的小缺失而无毒力，但不能将那些没有230-kb质粒或具有携带更广泛缺失的质粒的菌株转化为完全毒力。这表明质粒上有两个以上的毒力决定因子。因此，该序列包含一个遗传决定子，该决定子对于S中Pcr+表型的完全毒力和表达是必需的，但不是充分的。但在大肠杆菌中表达Pcr+表型是必需的和足够的。coli K-12。我们注意到，在230-kb质粒上还存在一些在E. coli K-12。尽管这些区域表达的Pcr+表型不如本研究中克隆的区域显著，但它们确实与其杂交。
A DNA sequence of about 1.0 kilobase (kb) derived from a 230-kb (140-megadalton) plasmid in a fully virulent Shigella flexneri 2a strain, YSH6000, was cloned into Escherichia coli K-12 by selecting for the ability to bind Congo red (Pcr+ phenotype). It was mapped and localized within the SalI restriction fragment F on the plasmid. This clone converted an S. flexneri strain which is avirulent as a result of a small deletion in the plasmid to full virulence but did not convert those without the 230-kb plasmid or with a plasmid bearing a more extensive deletion. This indicates that there are more than two virulence determinants on the plasmid. Thus, this sequence contains a genetic determinant which is essential but not sufficient for full virulence and expression of the Pcr+ phenotype in S. flexneri but is essential and sufficient for expression of the Pcr+ phenotype in E. coli K-12. We noted that there exist some other regions on the 230-kb plasmid which express the Pcr+ phenotype in E. coli K-12. Although these regions express the Pcr+ phenotype less markedly than the region cloned in the present study, they do hybridize with it.
DOI：
10.1073/pnas.81.19.6110
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
MEYER, TF;BILLYARD, E;SO, M
通讯作者：
SO, M
IS50 介导的逆转座。
影响因子：
5.6
作者：
Sasakawa,C;Berg,DE
通讯作者：
Berg,DE