A new model of cortical stroke in the rhesus macaque.
A new model of cortical stroke in the rhesus macaque.
复制标题
DOI：
10.1038/jcbfm.2009.43
复制
发表时间：
2009-06
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
灵长类动物模型是中风转化研究的重要工具，但据报道，它们产生可重复大小的皮质梗塞的能力不一致。在这里，我们报告了一种新的中风模型，在雄性恒河猴中使用经眼眶、可逆、两血管闭塞方法，产生一致且可重复的皮质梗塞。用血管夹闭塞右侧大脑中动脉（MCA）（眶额分支远端）和两条大脑前动脉（ACA）。 ACA 的双侧闭塞对于减少同侧皮质的侧支血流至关重要。诱导可逆性缺血 45、60 或 90 分钟（n=2/时间点），并评估梗塞体积和神经学结果。通过 T2 加权 MRI 确定，梗塞主要位于皮质，并且随着缺血持续时间的延长，梗塞面积增大。通过 2,3,5-三苯基氯化四唑 (TTC) 和甲酚紫染色测量的梗塞体积证实了 MRI 结果。随着闭塞时间的延长，神经功能缺损评分逐渐恶化。一部分动物 (n=5) 经历了 60 分钟的缺血，导致主要位于皮质的一致梗塞体积，这与神经功能缺损评分密切相关。这种方法为评估中风治疗干预措施提供了希望。
Primate models are essential tools for translational research in stroke, but are reportedly inconsistent in their ability to produce cortical infarcts of reproducible size. Here we report a new stroke model using a transorbital, reversible, two-vessel occlusion approach in male rhesus macaques that produces consistent and reproducible cortical infarcts. The right middle cerebral artery (MCA) (distal to the orbitofrontal branch), and both anterior cerebral arteries (ACA) were occluded with vascular clips. Bilateral occlusion of the ACA was critical for reducing collateral flow to the ipsilateral cortex. Reversible ischemia were induced for 45, 60, or 90 minutes (n=2/timepoint) and infarct volume and neurological outcome were evaluated. The infarcts were located predominantly in the cortex and increased in size with extended duration of ischemia determined by T2-weighted MRI. Infarct volume measured by 2,3,5-triphenyl tetrazolium chloride (TTC) and cresyl violet staining corroborated MRI results. Neurological deficit scores worsened gradually with longer occlusion times. A subset of animals (n=5) underwent 60 minutes of ischemia resulting in consistent infarct volumes primarily located to the cortex which correlated well with neurological deficit scores. This approach offers promise for evaluating therapeutic interventions in stroke.
登录
查看更多内容
影响因子：
4.1
作者：
JONES, TH;MORAWETZ, RB;OJEMANN, RG
通讯作者：
OJEMANN, RG
影响因子：
3
作者：
Maeda, M;Takamatsu, H;Matsuoka, N
通讯作者：
Matsuoka, N
影响因子：
2.4
作者：
DeGraba, TJ;Pettigrew, LC
通讯作者：
Pettigrew, LC
影响因子：
2.5
作者：
Liu, HP;Xin, LX;Tan, YH
通讯作者：
Tan, YH
影响因子：
1.9
作者：
Mack, WJ;Komotar, RJ;Connolly, ES
通讯作者：
Connolly, ES