Staphylococcus aureus enterotoxins induce IL-8 secretion by human nasal epithelial cells.
Staphylococcus aureus enterotoxins induce IL-8 secretion by human nasal epithelial cells.
复制标题
DOI：
10.1186/1465-9921-7-115
复制
发表时间：
2006-09-04
影响因子：
5.8
通讯作者：
Skibinski G
中科院分区：
文献类型：
--
作者：
O'Brien GJ;Riddell G;Elborn JS;Ennis M;Skibinski G
Staphylococcus aureus produces a set of proteins which act both as superantigens and toxins. Although their mode of action as superantigens is well understood, little is known about their effects on airway epithelial cells. To investigate this problem, primary nasal epithelial cells derived from normal and asthmatic subjects were stimulated with staphylococcal enterotoxin A and B (SEA and SEB) and secreted (supernatants) and cell-associated (cell lysates) IL-8, TNF-α, RANTES and eotaxin were determined by specific ELISAs. Non-toxic concentrations of SEA and SEB (0.01 μg/ml and 1.0 μg/ml) induced IL-8 secretion after 24 h of culture. Pre-treatment of the cells with IFN-γ (50 IU/ml) resulted in a further increase of IL-8 secretion. In cells from healthy donors pretreated with IFN-γ, SEA at 1.0 μg/ml induced release of 1009 pg/ml IL-8 (733.0–1216 pg/ml, median (range)) while in cells from asthmatic donors the same treatment induced significantly higher IL-8 secretion – 1550 pg/ml (1168.0–2000.0 pg/ml p = 0.04). Normal cells pre-treated with IFN-γ and then cultured with SEB at 1.0 μg/ml released 904.6 pg/ml IL-8 (666.5–1169.0 pg/ml). Cells from asthmatics treated in the same way produced significantly higher amounts of IL-8 – 1665.0 pg/ml (1168.0–2000.0 pg/ml, p = 0.01). Blocking antibodies to MHC class II molecules added to cultures stimulated with SEA and SEB, reduced IL-8 secretion by about 40% in IFN-γ unstimulated cultures and 75% in IFN-γ stimulated cultures. No secretion of TNF-α, RANTES and eotaxin was noted. Staphylococcal enterotoxins may have a role in the pathogenesis of asthma.
登录
查看更多内容
影响因子：
4.4
作者：
Fan, XJ;Gunasena, H;Reyes, VE
通讯作者：
Reyes, VE
影响因子：
3.1
作者：
HARRIS, TO;GROSSMAN, D;BETLEY, MJ
通讯作者：
BETLEY, MJ
DOI：
10.1073/pnas.92.14.6294
发表时间：
1995-07-03
影响因子：
11.1
作者：
BEHARKA, AA;IANDOLO, JJ;CHAPES, SK
通讯作者：
CHAPES, SK
影响因子：
3.5
作者：
Hu, DL;Suga, S;Nakane, A
通讯作者：
Nakane, A
DOI：
10.1164/ajrccm/147.2.291
发表时间：
1993-02-01
期刊：
AMERICAN REVIEW OF RESPIRATORY DISEASE
影响因子：
--
作者：
CEMBRZYNSKANOWAK, M;SZKLARZ, E;TEODORCZYKINJEYAN, JA
通讯作者：
TEODORCZYKINJEYAN, JA