Identification of a fish specific chemokine CXCL_F2 in large yellow croaker (Larimichthys crocea) reveals its primitive chemotactic function.
Identification of a fish specific chemokine CXCL_F2 in large yellow croaker (Larimichthys crocea) reveals its primitive chemotactic function.
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大黄鱼 (Larimichthys crocea) 中鱼类特异性趋化因子 CXCL_F2 的鉴定揭示了其原始趋化功能
DOI：
10.1016/j.fsi.2016.10.012
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发表时间：
2016-12
期刊：
影响因子：
--
通讯作者：
Chen Xinhua
中科院分区：
文献类型：
--
作者：
Zhou Shimin;Mu Yinnan;Liu Yingdi;Ao Jingqun;Chen Xinhua
趋化因子是在炎症和正常生理条件下调节免疫细胞迁移的细胞因子超家族。目前，一些鱼类特异性的CXC趋化因子，命名为CXCL_F1-5，已经在一些物种中被鉴定出来。然而，对其功能特征的了解仍然有限。本研究从大黄鱼（Larimichthys crocea）中鉴定出一种鱼类特异性趋化因子CXCL_F2 （LycCXCL_F2）。LycCXCL_F2的开放阅读框（ORF）长348个核苷酸，编码115个氨基酸（aa）的蛋白。推断的LycCXCL_F2蛋白含有一个20-aa的信号肽和一个95-aa的成熟多肽。系统发育分析表明，LycCXCL_F2属于由CXCL_F2序列组成的主进化支，从CXCL_F1和CXCL_F3-5亚群中分离出来。LycCXCL_F2 mRNA转录本在各组织中均有组成性表达，在脾脏和头肾中表达量最高。经灭活三价细菌疫苗刺激后，脾脏和头肾LycCXCL_F2 mRNA转录显著升高。重组LycCXCL_F2蛋白对大黄鱼外周血单核细胞、淋巴细胞和嗜酸性粒细胞有明显的趋化作用，但不能诱导巨噬细胞的呼吸爆发。这些结果表明，该鱼特异性CXC趋化因子LycCXCL_F2具有原始的趋化活性，可能在大黄鱼的免疫应答中发挥作用。
Chemokines are a superfamily of cytokines regulating immune cell migration under both inflammatory and normal physiological conditions. Currently, a number of fish specific CXC chemokines, named as CXCL_F1-5, have been identified in several species. However, understanding of their functional characteristics is still limited. In this study, we identified a fish specific chemokine CXCL_F2 (LycCXCL_F2) from large yellow croaker (Larimichthys crocea). The open reading frame (ORF) of LycCXCL_F2 is 348 nucleotides long, encoding a protein of 115 amino acids (aa). The deduced LycCXCL_F2 protein contains a 20-aa signal peptide and a 95-aa mature polypeptide. Phylogenetic analysis showed that LycCXCL_F2 fell into a major clade formed by CXCL_F2 sequences and was separated from CXCL_F1 and CXCL_F3-5 subgroups. LycCXCL_F2 mRNA transcript was constitutively expressed in various tissues, with the highest levels in spleen and head kidney. After stimulation with inactivated trivalent bacterial vaccines, LycCXCL_F2 mRNA transcription was significantly increased in both spleen and head kidney. Moreover, recombinant LycCXCL_F2 protein exhibited obvious chemotaxis to monocytes, lymphocytes and eosnophils of PBLs isolated from large yellow croaker, but could not induce the respiratory burst of macrophages. These results indicate that this fish specific CXC chemokine LycCXCL_F2 possesses primitive chemotactic activity and may play a role in immune response in large yellow croaker.
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