Polycaprolactone scaffold modified with galactosylated chitosan for hepatocyte culture
Polycaprolactone scaffold modified with galactosylated chitosan for hepatocyte culture
复制标题
半乳糖基化壳聚糖修饰的聚己内酯支架用于肝细胞培养
DOI：
10.1007/s13233-012-0056-8
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发表时间：
2012-03
影响因子：
2.4
通讯作者：
高长有
中科院分区：
文献类型：
--
作者：
高长有
一种合适的细胞外基质，可以维持长期增殖和肝细胞特异性功能的肝细胞是必不可少的体外生物人工肝（BAL）辅助系统和植入式细胞支架结构。本研究采用半乳糖化壳聚糖（GC）对聚己内酯（PCL）支架进行改性，以获得更好的生物活性和机械稳定性。通过明胶颗粒浸出的方法制备的PCL支架被水解以产生用于与GC的胺基反应的羧基，GC是通过将半乳糖接枝到壳聚糖上而合成的。结果表明，随着水解时间的延长，羧基含量逐渐增加，水解10 min后，羧基密度达到2.0×10− 5 mol/mg支架。GC的掺入量与COOH浓度呈正相关，水解时间为15 min时，GC的掺入量可达100 μg/mg支架。GC的掺入量对培养液的稳定性较好，可显著提高PCL支架在湿态下的抗压强度。在表面上具有半乳糖配体的PCL支架可以更好地被肝细胞识别，并且显示出更好的细胞活力、球状体形成和长期维持肝脏特异性功能，例如白蛋白分泌。
A suitable extracellular matrix that can maintain long-term proliferation and liver-specific functions of hepatocytes is essential for both extracorporeal bioartificial liver (BAL) assist systems and implantable cell-scaffold constructs. In this work, a polycaprolactone (PCL) scaffold was modified with galactosylated chitosan (GC) to achieve better bioactivity and mechanical stability. The PCL scaffold, prepared by a method of gelatin particle leaching, was hydrolyzed to produce carboxylic groups that were utilized to react with the amine groups of the GC, which was synthesized by grafting the galactose to chitosan. Results showed that the content of carboxylic groups was increased initially with the hydrolysis time until 10 min, at which a COOH density of 2.0×10−5mol/mg scaffold was reached. The incorporated GC amount showed a positive relationship with the COOH density and finally reached a 100 μg/mg scaffold with a hydrolysis time of 15 min. The incorporated GC was rather stable against incubation in the medium, and could significantly enhance the compression strength of the PCL scaffold in a wet state. With the galactose ligands on the surface, the PCL scaffold could be better recognized by hepatocytes, and show better cell viability, spheroid formation and long-term maintenance of liver-specific functions such as albumin secretion.
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影响因子：
14
作者：
Gotoh, Y;Niimi, S;Miyashita, T
通讯作者：
Miyashita, T
影响因子：
14
作者：
X. Chu;Xiao-lei Shi;Zhangqi Feng;Jin-yang Gu;Hai-Yun Xu;Yue Zhang;Z. Gu;Yi-tao Ding
通讯作者：
X. Chu;Xiao-lei Shi;Zhangqi Feng;Jin-yang Gu;Hai-Yun Xu;Yue Zhang;Z. Gu;Yi-tao Ding
影响因子：
14
作者：
Huang, Hongyun;Oizumi, Shunsuke;Sakai, Yasuyuki
通讯作者：
Sakai, Yasuyuki
影响因子：
1.5
作者：
Kulig, KM;Vacanti, JR
通讯作者：
Vacanti, JR
影响因子：
3.6
作者：
G. Hamilton;Summer L Jolley;D. Gilbert;James D. Coon;Scott A. Barros;E. LeCluyse
通讯作者：
G. Hamilton;Summer L Jolley;D. Gilbert;James D. Coon;Scott A. Barros;E. LeCluyse