CD14+CD16+ and CD14+CD163+ monocyte subpopulations in kidney allograft transplantation.
CD14+CD16+ and CD14+CD163+ monocyte subpopulations in kidney allograft transplantation.
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DOI：
10.1186/1471-2172-15-4
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发表时间：
2014-02-06
期刊：
影响因子：
3
通讯作者：
Striz I
中科院分区：
文献类型：
--
作者：
Sekerkova A;Krepsova E;Brabcova E;Slatinska J;Viklicky O;Lanska V;Striz I
单核细胞代表根据膜抗原的表达水平细分的异质细胞群。单核细胞的促炎（中间/非经典）亚群由 CD16 的表达定义。 CD163 似乎典型地优先由免疫抑制性单核细胞表达。我们研究的目的是评估 71 名同种异体肾移植患者中单核细胞亚群的分布。在规定的时间点通过流式细胞术评估表型。肾移植后外周CD14+CD16+单核细胞的比例立即下调，巴利昔单抗治疗部分减弱了这种趋势。 CD14+CD16+亚群的短暂下调在两个月内调整至基础值。大多数患者的肾移植后早期，CD14+CD163+单核细胞的比例短暂上调，并在第一个月内保持较高水平。在 ATG 治疗的患者中，CD14+CD163+单核细胞的扩增被延迟，但其上调持续时间更长。体外数据显示ATG和甲泼尼龙对CD16和CD163分子的表达有直接影响，而巴利昔单抗不影响培养的单核细胞的表型。根据我们的数据，我们假设肾同种异体移植与单核细胞亚群（CD14+CD16+和CD14+CD163+）的调节有关，部分受到所使用的免疫抑制方案的影响。
Monocytes represent a heterogeneous population of cells subdivided according to the expression level of membrane antigens. A pro-inflammatory (intermediate/nonclassical) subpopulation of monocytes is defined by expression of CD16. CD163 seems to be characteristically preferentially expressed by immunosuppressive monocytes. The aim of our study was to evaluate the distribution of monocyte subpopulations in 71 patients with kidney allograft transplantation. The phenotype was evaluated by flow cytometry in defined time points. The proportions of peripheral CD14+CD16+ monocytes were downregulated immediately after the kidney transplantation and basiliximab treatment partially attenuated this trend. The transient downregulation of the CD14+CD16+ subpopulation was adjusted to basal values in two months. The proportions of CD14+CD163+ monocytes were transiently upregulated early after the kidney transplantation and remained higher during the first month in most patients. In ATG treated patients, the expansion of CD14+CD163+ monocytes was delayed but their upregulation lasted longer. In vitro data showed the direct effect of ATG and methylprednisolone on expression of CD16 and CD163 molecules while basiliximab did not affect the phenotype of cultured monocytes. We assume from our data that kidney allograft transplantation is associated with modulation of monocyte subpopulations (CD14+CD16+ and CD14+CD163+) partially affected by an immunosuppressive regime used.
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