Direct binding of three tight junction-associated MAGUKs, ZO-1, ZO-2, and ZO-3, with the COOH termini of claudins.
Direct binding of three tight junction-associated MAGUKs, ZO-1, ZO-2, and ZO-3, with the COOH termini of claudins.
复制标题
三个紧密连接相关的Maguks ZO-1，ZO-2和ZO-3与Claudins的Cooh Termini直接结合。
DOI：
10.1083/jcb.147.6.1351
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发表时间：
1999-12-13
期刊：
影响因子：
--
通讯作者：
Tsukita S
中科院分区：
文献类型：
--
作者：
Itoh M;Furuse M;Morita K;Kubota K;Saitou M;Tsukita S
ZO-1、ZO-2和ZO-3含有三个PDZ结构域（PDZ 1至-3），集中在上皮细胞的紧密连接（TJ）处。TJ链主要由两种不同类型的四跨膜蛋白组成，闭合蛋白和封闭蛋白，据报道闭合蛋白直接结合ZO-1/ZO-2/ZO-3。然而，在闭合蛋白缺陷的肠上皮细胞中，ZO-1/ZO-2/ZO-3仍然被募集到TJ。然后我们研究了ZO-1/ZO-2/ZO-3和claudins之间可能的相互作用。ZO-1、ZO-2和ZO-3在体外通过它们的PDZ 1结构域结合到claudin-1至claudin-8的COOH-末端YV序列。然后，将claudin-1或-2转染到表达ZO-1但不表达ZO-2或ZO-3的L成纤维细胞中。紧密连接蛋白-1和-2集中在细胞-细胞边界的一个精心设计的网络模式，内源性ZO-1招募。当进一步转染ZO-2或ZO-3时，两者都与内源性ZO-1一起被募集到基于密蛋白的网络中。详细的分析表明，ZO-2和ZO-3通过PDZ 2（ZO-2或ZO-3）/PDZ 2（内源性ZO-1）和PDZ 1（ZO-2或ZO-3）/COOH末端YV（claudins）相互作用被招募到基于claudin的网络中。在良好的一致性，ZO-1/ZO-2/ZO-3的PDZ 1和PDZ 2结构域也被招募到基于紧密连接蛋白的TJ，当引入培养的上皮细胞。TJ斑块结构的可能的分子结构进行了讨论。
ZO-1, ZO-2, and ZO-3, which contain three PDZ domains (PDZ1 to -3), are concentrated at tight junctions (TJs) in epithelial cells. TJ strands are mainly composed of two distinct types of four-transmembrane proteins, occludin, and claudins, between which occludin was reported to directly bind to ZO-1/ZO-2/ZO-3. However, in occludin-deficient intestinal epithelial cells, ZO-1/ZO-2/ZO-3 were still recruited to TJs. We then examined the possible interactions between ZO-1/ZO-2/ZO-3 and claudins. ZO-1, ZO-2, and ZO-3 bound to the COOH-terminal YV sequence of claudin-1 to -8 through their PDZ1 domains in vitro. Then, claudin-1 or -2 was transfected into L fibroblasts, which express ZO-1 but not ZO-2 or ZO-3. Claudin-1 and -2 were concentrated at cell–cell borders in an elaborate network pattern, to which endogenous ZO-1 was recruited. When ZO-2 or ZO-3 were further transfected, both were recruited to the claudin-based networks together with endogenous ZO-1. Detailed analyses showed that ZO-2 and ZO-3 are recruited to the claudin-based networks through PDZ2 (ZO-2 or ZO-3)/PDZ2 (endogenous ZO-1) and PDZ1 (ZO-2 or ZO-3)/COOH-terminal YV (claudins) interactions. In good agreement, PDZ1 and PDZ2 domains of ZO-1/ZO-2/ZO-3 were also recruited to claudin-based TJs, when introduced into cultured epithelial cells. The possible molecular architecture of TJ plaque structures is discussed.
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DOI：
10.1083/jcb.121.3.491
发表时间：
1993-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Itoh M;Nagafuchi A;Yonemura S;Kitani-Yasuda T;Tsukita S;Tsukita S
通讯作者：
Tsukita S
影响因子：
64.8
作者：
KIM, E;NIETHAMMER, M;SHENG, M
通讯作者：
SHENG, M
紧密连接链内和之间异质claudin物种相互作用的方式。
影响因子：
7.8
作者：
Furuse, M;Sasaki, H;Tsukita, S
通讯作者：
Tsukita, S
DOI：
10.1083/jcb.115.5.1449
发表时间：
1991-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Itoh M;Yonemura S;Nagafuchi A;Tsukita S;Tsukita S
通讯作者：
Tsukita S
单个基因产物Claudin -1或-2，在成纤维细胞中重构紧密结合的连接链和新兵。
影响因子：
7.8
作者：
Furuse, M;Sasaki, H;Fujimoto, K;Tsukita, S
通讯作者：
Tsukita, S