Dendro-somatic synaptic inputs to ganglion cells contradict receptive field and connectivity conventions in the mammalian retina.
Dendro-somatic synaptic inputs to ganglion cells contradict receptive field and connectivity conventions in the mammalian retina.
复制标题
DOI：
10.1016/j.cub.2021.11.005
复制
发表时间：
2022-01-24
期刊：
影响因子：
--
通讯作者：
Diamond JS
中科院分区：
文献类型：
--
作者：
Grimes WN;Sedlacek M;Musgrove M;Nath A;Tian H;Hoon M;Rieke F;Singer JH;Diamond JS
视网膜神经元的形态对其生理功能有很大影响。神经节细胞（GC）树突在内丛状层（IPL）的不同层次上分叉，因此响应光增量（ON）或减量（OFF）的神经节细胞接受适当的兴奋输入。这种垂直分层规定了响应极性，并确保了细胞类型之间的一致连接，而GC树突状乔木的横向范围通常决定了接受野（RF）的大小。在这里，我们发现老鼠视网膜中的电路与这些惯例相矛盾。所有amacrine细胞都是中间神经元，通过将ON层的兴奋性输入传递到OFF层的抑制性输出来介导“交叉”抑制。然而，超微结构和生理分析表明，一些ai对OFF GC的胞体和ON层的近端树突具有强大的抑制作用，使得这些GC的抑制rf小于它们的树突乔木。这种OFF通路完全避开了IPL的OFF区域，挑战了视网膜电路的几个原则。这些结果还表明，在单个神经元群体中，亚细胞突触组织可以根据它们与潜在突触后靶点的接近程度而变化。所有的amacrine细胞对off反应的视网膜回路提供交叉抑制。在这里，Grimes等人表明，当aii细胞与OFFα神经节细胞体在空间上重叠时，aii的突触组织发生改变。因此，所有突触组织都是异质的，依赖于与特定突触后伙伴的接近程度。
The morphology of retinal neurons strongly influences their physiological function. Ganglion cell (GC) dendrites ramify in distinct strata of the inner plexiform layer (IPL) so that GCs responding to light increments (ON) or decrements (OFF) receive appropriate excitatory inputs. This vertical stratification prescribes response polarity and ensures consistent connectivity between cell types, whereas the lateral extent of GC dendritic arbors typically dictates receptive field (RF) size. Here, we identify circuitry in mouse retina that contradicts these conventions. AII amacrine cells are interneurons understood to mediate “cross-over” inhibition by relaying excitatory input from the ON layer to inhibitory outputs in the OFF layer. Ultrastructural and physiological analyses show, however, that some AIIs deliver powerful inhibition to OFF GC somas and proximal dendrites in the ON layer, rendering the inhibitory RFs of these GCs smaller than their dendritic arbors. This OFF pathway, avoiding entirely the OFF region of the IPL, challenges several tenets of retinal circuitry. These results also indicate that subcellular synaptic organization can vary within a single population of neurons according to their proximity to potential postsynaptic targets. AII amacrine cells provide crossover inhibition to OFF-responding retinal circuits. Here, Grimes, et al. show that the synaptic organization of AIIs is altered when the cells overlap in space with OFFα ganglion cell somas. AII synaptic organization is, therefore, heterogeneous and depends on proximity to specific postsynaptic partners.
登录
查看更多内容
影响因子：
16.2
作者：
Balakrishnan V;Puthussery T;Kim MH;Taylor WR;von Gersdorff H
通讯作者：
von Gersdorff H
影响因子：
5.5
作者：
CLELAND, BG;LEVICK, WR;WASSLE, H
通讯作者：
WASSLE, H
影响因子：
64.8
作者：
Chavez, Andres E.;Singer, Joshua H.;Diamond, Jeffrey S.
通讯作者：
Diamond, Jeffrey S.
影响因子：
64.8
作者：
Ding H;Smith RG;Poleg-Polsky A;Diamond JS;Briggman KL
通讯作者：
Briggman KL
影响因子：
1.9
作者：
Demb, Jonathan B.;Singer, Joshua H.
通讯作者：
Singer, Joshua H.