Heat Shock Protein 90α Is a Potential Serological Biomarker of Acute Rejection after Renal Transplantation.
Heat Shock Protein 90α Is a Potential Serological Biomarker of Acute Rejection after Renal Transplantation.
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DOI：
10.1371/journal.pone.0162942
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Masumori N
中科院分区：
文献类型：
--
作者：
Maehana T;Tanaka T;Kitamura H;Fukuzawa N;Ishida H;Harada H;Tanabe K;Masumori N
热休克蛋白90 （HSP90）是一种与客户蛋白激活相关的分子伴侣，最近被报道在免疫反应中起重要作用。迄今为止，热休克蛋白90在实体器官移植中的作用尚不清楚。本研究的目的是评估血清HSP90α水平与器官和组织移植后急性同种异体移植排斥反应之间的关系，采用肾移植受体血清样本、体外抗体介导的排斥反应模型和小鼠皮肤移植。肾受体急性排斥反应（AR）时血清HSP90α水平明显高于无排斥反应的肾受体。在大多数AR病例中，治疗后血清HSP90降至基线水平。另一方面，慢性排斥反应、钙调磷酸酶抑制剂肾毒性或BK病毒肾病患者的血清HSP90α没有AR患者升高那么多。体外研究表明，补体攻击下与特异性抗hla IgG共培养的人主动脉内皮细胞上清中HSP90α浓度明显高于与非特异性IgG共培养的细胞上清。在接受皮肤移植的小鼠中，血清HSP90α在第一次移植被排斥时升高，在第二次移植被更严重排斥时进一步升高。结果表明，在器官和组织移植中，HSP90α通过AR引起的细胞损伤释放到血清中，它可能是一种新的生物标志物，有助于检测肾受体AR。
Heat shock protein 90 (HSP90), a molecular chaperone associated with the activation of client proteins, was recently reported to play an important role in immunologic reactions. To date, the role of HSP90 in solid organ transplantations has remained unknown. The aim of this study was to evaluate the relationship between serum HSP90α levels and acute allograft rejection after organ and tissue transplantation using serum samples from kidney allograft recipients, an in vitro antibody-mediated rejection model, and a murine skin transplantation. Serum HSP90α levels were significantly higher in kidney recipients at the time of acute rejection (AR) than in those with no evidence of rejection. In most cases with AR, serum HSP90 decreased to baseline after the treatment. On the other hand, serum HSP90α was not elevated as much in patients with chronic rejection, calcineurin inhibitor nephrotoxicity, or BK virus nephropathy as in AR patients. In vitro study showed that HSP90α concentration in the supernatant was significantly higher in the supernatant of human aortic endothelial cells cocultured with specific anti-HLA IgG under complement attack than in that of cells cocultured with nonspecific IgG. In mice receiving skin transplantation, serum HSP90α was elevated when the first graft was rejected and the level further increased during more severe rejection of the second graft. The results suggest that HSP90α is released into the serum by cell damage due to AR in organ and tissue transplantation, and it is potentially a new biomarker to help detect AR in kidney recipients.
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DOI：
10.1073/pnas.96.9.5159
发表时间：
1999-04-27
影响因子：
11.1
作者：
Birk, OS;Gur, SL;Cohen, IR
通讯作者：
Cohen, IR
影响因子：
1.5
作者：
Soltaninejad, Ehsan;Nicknam, Mohammad Hossein;Amirzargar, Aliakbar
通讯作者：
Amirzargar, Aliakbar
影响因子：
--
作者：
Whitesell, L;Cook, P
通讯作者：
Cook, P
影响因子：
3.7
作者：
Tukaj, Stefan;Kleszczynski, Konrad;Kasperkiewicz, Michael
通讯作者：
Kasperkiewicz, Michael
影响因子：
11.4
作者：
Li, Wei;Li, Yong;Woodley, David T.
通讯作者：
Woodley, David T.