Sensitive Determination of Amantadine in Microdialysis Samples from Rat Plasma by HPLC with Fluorescence Detection
Sensitive Determination of Amantadine in Microdialysis Samples from Rat Plasma by HPLC with Fluorescence Detection
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采用荧光检测 HPLC 灵敏测定大鼠血浆微透析样品中的金刚烷胺
DOI：
10.1080/10826076.2015.1087026
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发表时间：
2015-08
影响因子：
1.3
通讯作者：
You, Jinmao
中科院分区：
文献类型：
--
作者：
Zhang, Shijuan;Sheng, Cuncun;Zhao, Xian;You, Jinmao
建立了以10-甲基吖啶酮-2-磺酰氯（MASC）为柱前荧光标记剂测定大鼠血浆微透析样品中金刚烷胺的高效液相色谱（HPLC）方法。在金刚烷胺分子中引入具有大共轭结构和优良荧光特性的MASC，大大提高了金刚烷胺的高效液相色谱灵敏度。检出限为6.8 ng mL−1，只需30 μL样品即可进行药代动力学研究。线性范围为25 ~ 500 ng mL−1，相关系数为0.9989。该方法成功地应用于大鼠血浆微透析样品中金刚烷胺的测定。这也为氨基化合物的药代动力学研究显示了强大的潜力。
A new and sensitive high-performance liquid chromatography (HPLC) method with 10-methyl-acridone-2-sulfonyl chloride (MASC) as pre-column fluorescence labeling reagent was developed for the determination of amantadine in microdialysis samples from rat plasma. The HPLC sensitivity of amantadine was greatly enhanced by introducing MASC with large conjugated structure and excellent fluorescence property into the amantadine molecule. The limit of detection was 6.8 ng mL−1 and only 30 μL sample was needed for the pharmacokinetic study. The linearity range was 25–500 ng mL−1 with correlation coefficients of 0.9989. The proposed method was successfully applied to the determination of amantadine in microdialysis samples from rat plasma. It also shows powerful potential for the pharmacokinetic study of compounds with amino group.
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影响因子：
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