Recent trends in microdialysis sampling integrated with conventional and microanalytical systems for monitoring biological events: a review.

Recent trends in microdialysis sampling integrated with conventional and microanalytical systems for monitoring biological events: a review.
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DOI:
10.1016/j.aca.2009.07.064
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发表时间:
2009-09-28
影响因子:
6.2
通讯作者:
Lunte SM
Lunte SM
中科院分区:
化学1区
文献类型:
--
作者:
Nandi P;Lunte SM

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微透析(MD)是一种可用于监测体内和体外生物事件的采样技术。当它被耦合到一个分析系统,微透析可以提供近实时的信息,在细胞外空间或其他水性环境中的分析物的时间依赖性浓度变化。用于微透析样品分析的在线系统能够实现快速、选择性和灵敏的分析,同时保留时间信息。用于在线分析的分析方法包括液相色谱(LC)、毛细管(CE)和微芯片电泳以及流通式生物传感器装置。本文综述了微透析取样和在线分析系统,重点是在体内分析。影响分析频率的因素,因此,这些系统的时间分辨率也进行了讨论。
Microdialysis (MD) is a sampling technique that can be employed to monitor biological events both in vivo and in vitro. When it is coupled to an analytical system, microdialysis can provide near realtime information on the time-dependent concentration changes of analytes in the extracellular space or other aqueous environments. Online systems for the analysis of microdialysis samples enable fast, selective and sensitive analysis while preserving the temporal information. Analytical methods employed for online analysis include liquid chromatography (LC), capillary (CE) and microchip electrophoresis and flow-through biosensor devices. This review article provides an overview of microdialysis sampling and online analysis systems with emphasis on in vivo analysis. Factors that affect the frequency of analysis and, hence, the temporal resolution of these systems are also discussed.
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