Subcellular curvature at the perimeter of micropatterned cells influences lamellipodial distribution and cell polarity.
Subcellular curvature at the perimeter of micropatterned cells influences lamellipodial distribution and cell polarity.
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DOI：
10.1002/cm.20305
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发表时间：
2008-11
影响因子：
--
通讯作者：
Mrksich, Milan
中科院分区：
文献类型：
--
作者：
James, Jane;Goluch, Edgar D.;Hu, Huan;Liu, Chang;Mrksich, Milan
这篇论文使用的底物图案的形状具有明确的几何线索，以表征曲率对高转移的B16F10大鼠黑色素瘤细胞极化的影响。使用微接触印刷对衬底进行图案化，以在防止细胞粘连的背景上限定定义的形状和大小的粘附岛。附着在这些表面上的细胞对粘附岛周边处的局部曲率做出反应；凸面特征促进片状脂膜的组装，凹面特征促进应力细丝的组装。附着在矩形形状上的细胞显示出极化的细胞骨架，随着形状的长宽比而增加。将局部几何线索通过凹边或凸边与长宽比相结合的形状被用来理解形状对极化的相加效应。细胞极性对形状的依赖性是在改变肌动球蛋白收缩能力的小分子存在的情况下确定的，与具有弯曲边缘的形状相比，具有直边的形状对收缩能力的依赖程度更强。这项研究表明，细胞骨架在细胞外环境中对多种几何线索做出反应，从而调节细胞的极性。
This paper employs substrates that are patterned with shapes having well-defined geometric cues to characterize the influence of curvature on the polarization of highly metastatic B16F10 rat melanoma cells. Substrates were patterned using microcontact printing to define adhesive islands of defined shape and size on a background that otherwise prevents cell adhesion. Cells adherent to these surfaces responded to local curvature at the perimeter of the adhesive islands; convex features promoted the assembly of lamellipodia and concave features promoted the assembly of stress filaments. Cells adherent to rectangular shapes displayed a polarized cytoskeleton that increased with the aspect ratio of the shapes. Shapes that combined local geometric cues, by way of concave or convex edges, with aspect ratio were used to understand the additive effects of shape on polarization. The dependence of cell polarity on shape was determined in the presence of small molecules that alter actomyosin contractility and revealed a stronger dependence on contractility for shapes having straight edges, in contrast to those having curved edges. This study demonstrates that the cytoskeleton modulates cell polarity in response to multiple geometric cues in the extracellular environment.
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DOI：
10.3109/15419069809040295
发表时间：
1998-01-01
期刊：
CELL ADHESION AND COMMUNICATION
影响因子：
--
作者：
Liu, BP;Chrzanowska-Wodnicka, M;Burridge, K
通讯作者：
Burridge, K
影响因子：
3.3
作者：
Kolega, John
通讯作者：
Kolega, John
影响因子：
2.9
作者：
Madeja, Z;Szymkiewicz, I;Korohoda, W
通讯作者：
Korohoda, W
影响因子：
4.4
作者：
Zhang, M;Rao, PV
通讯作者：
Rao, PV
DOI：
10.1073/pnas.0408954102
发表时间：
2005-01-25
影响因子：
11.1
作者：
Jiang, XY;Bruzewicz, DA;Whitesides, GM
通讯作者：
Whitesides, GM