Aly and THO are required for assembly of the human TREX complex and association of TREX components with the spliced mRNA.
Aly and THO are required for assembly of the human TREX complex and association of TREX components with the spliced mRNA.
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Aly 和 THO 是人类 TREX 复合物组装以及 TREX 成分与剪接 mRNA 关联所必需的
DOI：
10.1093/nar/gks1188
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发表时间：
2013-01
影响因子：
14.9
通讯作者：
Cheng H
中科院分区：
文献类型：
--
作者：
Chi B;Wang Q;Wu G;Tan M;Wang L;Shi M;Chang X;Cheng H
已知mRNA输出复合物TREX（TREX）含有Aly、UAP56、Tex1和THO复合物，其中UAP56是TREX组装所需的。在这里，我们系统地研究了每个人TREX组件在TREX组装及其与mRNA的关联中的作用。我们发现Tex1基本上是THO复合物的一个亚基。Aly、THO和UAP 56都是TREX组装所需的，其中Aly直接与THO亚基THB2和THB5相互作用。Aly和THO均在将UAP 56连接至帽结合蛋白CBP 80中起作用。有趣的是，UAP56与剪接的mRNA而不是与前mRNA的关联需要Aly和THO。出乎意料的是，我们发现Aly和THO需要彼此才能与剪接的mRNA结合。与这些生化结果一致，类似于Aly和UAP 56，THO在mRNA输出中起关键作用。总之，我们提出，Aly，THO和UAP56形成一个高度整合的单位，与剪接的mRNA和功能的mRNA输出。
The mRNA export complex TREX (TREX) is known to contain Aly, UAP56, Tex1 and the THO complex, among which UAP56 is required for TREX assembly. Here, we systematically investigated the role of each human TREX component in TREX assembly and its association with the mRNA. We found that Tex1 is essentially a subunit of the THO complex. Aly, THO and UAP56 are all required for assembly of TREX, in which Aly directly interacts with THO subunits Thoc2 and Thoc5. Both Aly and THO function in linking UAP56 to the cap-binding protein CBP80. Interestingly, association of UAP56 with the spliced mRNA, but not with the pre-mRNA, requires Aly and THO. Unexpectedly, we found that Aly and THO require each other to associate with the spliced mRNA. Consistent with these biochemical results, similar to Aly and UAP56, THO plays critical roles in mRNA export. Together, we propose that Aly, THO and UAP56 form a highly integrated unit to associate with the spliced mRNA and function in mRNA export.
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影响因子：
16
作者：
Johnson, Sara Ann;Cubberley, Gabrielle;Bentley, David L.
通讯作者：
Bentley, David L.
影响因子：
10.5
作者：
Bruhn, L;Munnerlyn, A;Grosschedl, R
通讯作者：
Grosschedl, R
DOI：
10.1073/pnas.0400933101
发表时间：
2004-03-23
影响因子：
11.1
作者：
Ferraiuolo, MA;Lee, CS;Sonenberg, N
通讯作者：
Sonenberg, N
影响因子：
64.5
作者：
Cheng, Hong;Dufu, Kobina;Reed, Robin
通讯作者：
Reed, Robin
影响因子：
4.5
作者：
Custódio, N;Carvalho, C;Carmo-Fonseca, M
通讯作者：
Carmo-Fonseca, M