Transbilayer Movement of Various Phosphatidylcholine Species in Intact Human Erythrocytes
Transbilayer Movement of Various Phosphatidylcholine Species in Intact Human Erythrocytes
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完整人红细胞中各种磷脂酰胆碱的跨双层运动
DOI：
10.1002/jcb.240190209
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发表时间：
1982
影响因子：
4
通讯作者：
J. Op den Kamp
中科院分区：
文献类型：
--
作者：
Gerrit van Meer;J. Op den Kamp
用磷脂酰胆碱特异性磷脂交换蛋白将（14 C）标记的不同脂酰组成的磷脂酰胆碱导入完整的人红细胞。磷脂酶A2和鞘磷脂酶的组合水解，以证明原来所有新引入的磷脂酰胆碱居住在外层单层。随后，将红细胞在37°C下再孵育，并通过在不同孵育时间后应用磷脂酶的组合来监测引入的（14C）磷脂酰胆碱的再分布。在20%的天然红细胞磷脂酰胆碱被来自（14 C）胆碱标记的大鼠肝微粒体膜的磷脂酰胆碱替代的情况下，发现（14 C）微粒体磷脂酰胆碱的缓慢移位，具有10.8小时的跨双层平衡的半衰期。在大量红细胞磷脂酰胆碱的脂肪酰基组成保持不变的条件下，研究了（14 C）卵磷脂和（14 C）大豆磷脂酰胆碱。与探针不饱和度的增加相关，跨双层平衡的半衰期分别计算为26.9、12.8和8.1小时。
Phosphatidylcholine specific phospholipid exchange protein was used to introduce (14C)-labeled phosphatidylcholine of different fatty acyl compositions into the intact human erythrocyte. Hydrolysis by a combination of phospholipase A2 and sphingomyelinase was applied to prove that originally all newly introduced phosphatidylcholine resided in the outer monolayer. Subsequently the erythrocytes were reincubated at 37°C, and redistribution of the introduced (14C)phosphatidylcholine was monitored by applying the combination of phospholipases after different times of incubation. In the situation where 20% of the native erythrocyte phosphatidylcholine had been replaced by phosphatidylcholine from (14C)choline-labeled rat liver microsomal membranes, a slow translocation of the (14C)microsomal phosphatidylcholine was found, with a half-time of transbilayer equilibration of 10.8 hr. Furthermore, the transbilayer movement of probe amounts of (14C)dipalmitoyl-phosphatidylcholine, (14C)egg phosphatidylcholine and (14C)soybean phosphatidylcholine was studied under conditions whereby the fatty acyl composition of the bulk erythrocyte phosphatidylcholine remained unchanged. In correlation to the increasing unsaturation of the probe, half-times for the transbilayer equilibration were calculated to be 26.9, 12.8, and 8.1 hr, respectively.
影响因子：
6.5
作者：
W. R. Morrison;L. Smith
通讯作者：
W. R. Morrison;L. Smith