Expression of microRNA-122 contributes to apoptosis in H9C2 myocytes.
Expression of microRNA-122 contributes to apoptosis in H9C2 myocytes.
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DOI：
10.1111/j.1582-4934.2012.01577.x
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发表时间：
2012-11
影响因子：
5.3
通讯作者：
Dai S
中科院分区：
文献类型：
--
作者：
Huang X;Huang F;Yang D;Dong F;Shi X;Wang H;Zhou X;Wang S;Dai S
microRNA（miRNAs）可以在转录后调节基因表达和心脏发育。Pax-8基因敲除小鼠具有明显的心脏异常。本研究探讨了miRNAs在基因敲除小鼠心脏凋亡和发育中的调控作用。MicroRNA微阵列显示了Pax-8 −/−和Pax-8 +/−小鼠之间microRNA的差异表达，并通过实时PCR证实。miR-122在Pax-8 −/−小鼠中上调了1.92倍。Pax-8 −/−小鼠出现室间隔缺损，Pax-8 −/−小鼠左心室壁和室间隔的凋亡细胞数量增加。在H9C2心肌细胞中，用miR-122模拟物或miR-122抑制剂处理影响CCK-8的表达和Caspase-3的活性。miR-122在Pax-8 −/−小鼠心肌细胞中表达上调，可能参与凋亡基因表达和心脏发育缺陷的发病机制。
The microRNAs (miRNAs) can post-transcriptionally regulate gene expression and heart development. The Pax-8 gene knockout mice have apparent heart abnormalities. This study investigated the role of miRNAs in regulation of cardiac apoptosis and development in the knockout mice. MicroRNA microarrays demonstrated differential expression of microRNAs between Pax-8−/− and Pax-8+/− mice, confirmed by real-time PCR. The miR-122 was up-regulated by 1.92 folds in Pax-8−/− mice. There were ventricular septum defects in Pax-8−/− mice, and increased numbers of apoptotic cells in the left ventricular wall and interventricular septum in Pax-8−/− mice. In H9C2 myocytes, treatment with miR-122 mimics or miR-122 inhibitor affects the expression of CCK-8 and activity of Caspase-3. The miR-122 is up-regulated in the myocytes of Pax-8−/− mice and may participate in the apoptotic gene expression and pathogenesis of heart development defect.
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