Protocol for Analyzing Cell Cycle Phases of Polyphenic Wings Using Flow Cytometry.
Protocol for Analyzing Cell Cycle Phases of Polyphenic Wings Using Flow Cytometry.
复制标题
使用流式细胞术分析多酚翼细胞周期阶段的方案
DOI：
10.1016/j.xpro.2020.100080
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发表时间：
2020-09-18
期刊：
影响因子：
--
通讯作者：
Lin X
中科院分区：
文献类型：
--
作者：
Zhang Y;Lin X
破译多型组织的细胞周期阶段是理解多态性细胞机制的一个重要挑战。我们使用流式细胞术分析褐飞虱短翅和长翅的细胞周期阶段。这提供了不同翅膀形式的细胞周期停滞阶段的信息。该协议经修改后可应用于其他多苯昆虫和不同多苯组织的细胞周期阶段的分析。有关该协议的使用和执行的完整详细信息，请参阅。我们使用流式细胞术来分析翅膀的细胞周期阶段 应去除双联体并分析单细胞群 我们比较了短翼和长翼的细胞周期阶段 该协议可以应用于其他多型组织 破译多型组织的细胞周期阶段是理解多态性细胞机制的一个重要挑战。我们使用流式细胞术分析褐飞虱短翅和长翅的细胞周期阶段。这提供了不同翅膀形式的细胞周期停滞阶段的信息。该协议经修改后可应用于其他多苯昆虫和不同多苯组织的细胞周期阶段的分析。
Deciphering cell cycle phases of polyphenic tissues is an important challenge in understanding the cellular mechanism of polymorphism. We use flow cytometry to analyze cell cycle phases of short wings and long wings of the brown planthopper. This provides information on the arresting cell cycle phases in different wing forms. The protocol could be applied to analysis of the cell cycle phases of other polyphenic insects and in different polyphenic tissues after modification. For complete details on the use and execution of this protocol, please refer to. We use flow cytometry to analyze cell cycle phases of wings Doublets should be removed and the single cell population was analyzed We compared the arresting cell cycle phases of short and long wings The protocol could be applied to other polyphenic tissues Deciphering cell cycle phases of polyphenic tissues is an important challenge in understanding the cellular mechanism of polymorphism. We use flow cytometry to analyze cell cycle phases of short wings and long wings of the brown planthopper. This provides information on the arresting cell cycle phases in different wing forms. The protocol could be applied to analysis of the cell cycle phases of other polyphenic insects and in different polyphenic tissues after modification.
细胞周期进程决定多酚昆虫褐飞虱翅膀的形态
影响因子：
5.8
作者：
Lin, Xinda;Gao, Han;Lavine, Laura Corley
通讯作者：
Lavine, Laura Corley