Green separation of bromelain in food sample with high retention of enzyme activity using recyclable aqueous two-phase system containing a new synthesized thermo-responsive copolymer and salt.
Green separation of bromelain in food sample with high retention of enzyme activity using recyclable aqueous two-phase system containing a new synthesized thermo-responsive copolymer and salt.
复制标题
使用含有新型合成热响应性共聚物和盐的可回收水性两相系统，对食品样品中的菠萝蛋白酶进行绿色分离，并高度保留酶活性。
DOI：
10.1016/j.foodchem.2019.01.005
复制
发表时间：
2019-06
期刊：
影响因子：
8.8
通讯作者：
Ni Liang
中科院分区：
文献类型：
--
作者：
Wang Lei;Li Wenxuan;Liu Yuanyuan;Zhi Wenjing;Han Juan;Wang Yun;Ni Liang
High cost and enzyme deactivation in purification process are the two main obstacles for the use of enzyme as green catalyst. The objective of this work was to overcome these limitations by developing a cost-effective aqueous two-phase system (ATPS) for efficient purification of enzymes with remarkable separation efficiency and high retention of enzyme activity. The ATPS was formed by thermo-responsive block copolymer PEG113-b-PNIPAM149and salt as phase-forming components combining economy, recovery and sustainability. This strategy fabricated block copolymer with specified molecular weight and low LCST, which not only achieved better phase splitting but also ensured easy recycling for block copolymer. The developed ATPS demonstrated excellent extraction and biocompatibility for bromelain in real sample with 94.87% separation efficiency and 77.06% activity, which were remarkably higher than those obtained in EOPOEO-based ATPS. The recycling of copolymer was introduced to minimize cost, with recovery efficiency of 90% in the five cycles.
登录
查看更多内容
DOI：
10.1039/c7gc03880a
发表时间：
2018-03-21
期刊：
Green chemistry : an international journal and green chemistry resource : GC
影响因子：
--
作者：
Ferreira AM;Passos H;Okafuji A;Tavares APM;Ohno H;Freire MG;Coutinho JAP
通讯作者：
Coutinho JAP
DOI：
10.1016/j.jct.2017.01.009
发表时间：
2017-05
期刊：
The Journal of Chemical Thermodynamics
影响因子：
--
作者：
Xiana Rico-Castro;Marlen González-Amado;A. Soto;O. Rodríguez
通讯作者：
Xiana Rico-Castro;Marlen González-Amado;A. Soto;O. Rodríguez
热敏聚合物L61与盐水两相体系的浊点和液液平衡行为
影响因子：
3.3
作者：
Rao Wenwei;Wang Yun;Han Juan;Wang Lei;Chen Tong;Liu Yan;Ni Liang
通讯作者：
Ni Liang
DOI：
10.1016/j.jct.2017.07.028
发表时间：
2017-12
期刊：
The Journal of Chemical Thermodynamics
影响因子：
--
作者：
Cher Pin Song;R. Ramanan;R. Vijayaraghavan;D. Macfarlane;E. Chan;J. Coutinho;Luis Fernández;C. Ooi
通讯作者：
Cher Pin Song;R. Ramanan;R. Vijayaraghavan;D. Macfarlane;E. Chan;J. Coutinho;Luis Fernández;C. Ooi
DOI：
10.1016/j.chroma.2016.10.016
发表时间：
2016-11
期刊：
Journal of chromatography. A
影响因子：
--
作者：
Chengning Xu;Wenying Dong;J. Wan;Xuejun Cao
通讯作者：
Chengning Xu;Wenying Dong;J. Wan;Xuejun Cao