Protective effects of glaucocalyxin A on the airway of asthmatic mice.
Protective effects of glaucocalyxin A on the airway of asthmatic mice.
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青萼素A对哮喘小鼠气道的保护作用
DOI：
10.1515/med-2022-0513
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发表时间：
2022
期刊：
影响因子：
2.1
通讯作者：
Yan, Guanghai
中科院分区：
文献类型：
--
作者：
Chen, Si;Piao, Ying;Song, Yilan;Wang, Zhiguang;Jiang, Jingzhi;Piao, Yihua;Li, Li;Xu, Chang;Li, Liangchang;Chi, Yongxue;Jin, Guihua;Yan, Guanghai
本研究从炎症介质、Th1/Th2亚群失衡、Toll样受体4（TLR4）/NF-κ B信号通路等方面探讨蓝萼甲素（GLA）对哮喘小鼠气道的保护作用。采用苏木精-伊红/高碘酸-希夫染色法观察肺组织病理变化。采用酶联免疫吸附法检测支气管肺泡灌洗液中炎性细胞因子的含量。蛋白质表达水平用Western blot、免疫组化和免疫荧光法检测。体内研究表明，GLA治疗可降低卵清蛋白（OVA）诱导的哮喘小鼠模型的气道高反应性，减少炎性细胞的分泌，抑制杯状细胞的增殖，降低IL-4、IL-5和IL-13的水平，增加IFN-γ和IL-12的含量。GLA还能抑制哮喘小鼠模型中TLR 4、MyD 88、TRAF 6和NF-κ B的蛋白表达。进一步的体外研究表明，GLA可抑制脂多糖刺激的RAW 264. 7巨噬细胞中NF-κ B、p-I κ B α、肿瘤坏死因子-α、IL-6和IL-1 β的表达，并阻断NF-κ B的核转移。结论：GLA可抑制OVA诱导的哮喘小鼠炎症反应，抑制LPS诱导的RAW264. 7巨噬细胞炎症因子的释放，其机制可能与抑制TLR4/NF-κ B信号通路有关。
The aim of this study is to investigate the protective effects of glaucocalyxin A (GLA) on airways in mouse models of asthma, concerning the inflammatory mediators, Th1/Th2 subgroup imbalance, and Toll-like receptor 4 (TLR4)/NF-κB signaling pathway. Hematoxylin and eosin/periodic acid–Schiff staining was used to observe the pathological changes in lung tissues. Inflammatory cytokine contents in the bronchoalveolar lavage fluid were detected by enzyme-linked immunosorbent assay. Protein expression levels were detected with Western blot, immunohistochemistry, and immunofluorescence. In vivo studies showed that, in ovalbumin (OVA)-induced asthmatic mouse models, the GLA treatments reduced the airway hyperresponsiveness and the secretion of inflammatory cells, declined the proliferation of goblet cells, decreased the levels of IL-4, IL-5, and IL-13, and increased the contents of interferon-γ and IL-12. Moreover, GLA inhibited the protein expression levels of TLR4, MyD88, TRAF6, and NF-κB in OVA-induced asthmatic mouse models. Further in vitro studies showed that GLA inhibited the expression of NF-κB, p-IκBα, tumor necrosis factor-α, IL-6, and IL-1β and blocked the nuclear transfer of NF-κB in lipopolysaccharide-stimulated RAW264.7 macrophages. Conclusively, GLA can inhibit the inflammatory responses in OVA-induced asthmatic mice and inhibit the release of inflammatory factors in LPS-induced RAW264.7 macrophages, which may be related to the inhibition of TLR4/NF-κB signaling pathway.
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DOI：
10.4168/aair.2016.8.2.132
发表时间：
2016-03
期刊：
Allergy, asthma & immunology research
影响因子：
--
作者：
Luo X;Hong H;Tang J;Wu X;Lin Z;Ma R;Fan Y;Xu G;Liu D;Li H
通讯作者：
Li H
DOI：
10.1155/2021/6644751
发表时间：
2021
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Piao Y;Jiang J;Wang Z;Wang C;Jin S;Li L;Li L;Piao H;Jin Z;Zhu L;Yan G
通讯作者：
Yan G
影响因子：
2.7
作者：
Chen, Tianyi;Liang, Weibo;Zhang, Lin
通讯作者：
Zhang, Lin
影响因子：
6
作者：
Han, Meihua;Li, Zhitao;Wang, Xiangtao
通讯作者：
Wang, Xiangtao
影响因子：
5.6
作者：
Hou, Xiaorong;Xu, Guang;Bai, Zhaofang
通讯作者：
Bai, Zhaofang