A novel role of Fas in delaying cellular senescence.
A novel role of Fas in delaying cellular senescence.
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DOI：
10.1016/j.heliyon.2023.e13451
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发表时间：
2023-03
期刊：
影响因子：
4
通讯作者：
Huang, Gonghua
中科院分区：
文献类型：
--
作者：
Saha, Chaitrali;Li, Jingyu;Sun, Xuerong;Liu, Xinguang;Huang, Gonghua
Fas-mediated apoptosis is a major player of many physiological and pathological cellular processes. Fas-regulated immune regulation exhibits either the beneficial or the harmful effects which is associated with the onset or development of immune disorders. Alterations in apoptosis may contribute to age-associated changes. However, the role of apoptosis in the ageing process remains ambiguous. Here we demonstrated Fas signaling-mediated premature senescence in young mouse embryonic fibroblast (MEF) cells. Activated Fas signaling by agonist Jo-2 resulted in declined senescence in young and aged MEFs. Premature senescence induced the early activation of senescence markers, including the increase in the percentage of SA-β-galactosidase (SA-β-gal) cells, the induction of p53 phosphorylation, and the enhanced expression of p16 and p21 protein and elevated IL-6 pro-inflammatory cytokine in the absence of Fas. The elevated production of reactive oxygen species (ROS) in Fas-deficient MEFs was associated with dysfunctional mitochondria. Further, we determined that the known ROS scavenger NAC (N-acetyl-l-cysteine) could reverse the process of premature senescence in absence of Fas. Therefore, this study signifies a novel role of Fas in the control of cellular senescence. Absence of Fas signaling led to premature cellular senescence, which was significantly reduced by Fas agonist Jo-2 . Young Fas knock out (FasKO) MEF cells exhibit enhanced SA-β-gal activity, p16, p21, p53, pP38 and IGFBP5. Young FasKO cells display elevated inflammatory IL-6. Young FasKO MEF cells produced significantly higher ROS and defect in mitochondrial function. ROS scavenger NAC (N-acetyl-l-cysteine) could reverse the process of premature aging in absence of Fas.
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影响因子：
3.7
作者：
Balestro E;Calabrese F;Turato G;Lunardi F;Bazzan E;Marulli G;Biondini D;Rossi E;Sanduzzi A;Rea F;Rigobello C;Gregori D;Baraldo S;Spagnolo P;Cosio MG;Saetta M
通讯作者：
Saetta M
影响因子：
3.5
作者：
Chapman, James;Fielder, Edward;Passos, Joao F.
通讯作者：
Passos, Joao F.
影响因子：
4.8
作者：
Oparka, Monika;Walczak, Jaroslaw;Wieckowski, Mariusz R.
通讯作者：
Wieckowski, Mariusz R.
衰老中的细胞衰老：从机制到治疗机会。
DOI：
10.1038/s41580-020-00314-w
发表时间：
2021-03
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
Di Micco R;Krizhanovsky V;Baker D;d'Adda di Fagagna F
通讯作者：
d'Adda di Fagagna F
影响因子：
9.8
作者：
Coppé JP;Patil CK;Rodier F;Sun Y;Muñoz DP;Goldstein J;Nelson PS;Desprez PY;Campisi J
通讯作者：
Campisi J