Studies in plaque pathogenicity. I. Plaque collection and limulus lysate screening of adherent and loosely adherent plaque.
Studies in plaque pathogenicity. I. Plaque collection and limulus lysate screening of adherent and loosely adherent plaque.
复制标题
斑块致病性的研究。
DOI：
10.1111/j.1600-0765.1978.tb00150.x
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发表时间：
1978
影响因子：
3.5
通讯作者：
K. Siegel
中科院分区：
文献类型：
--
作者：
D. Fine;L. Tabak;H. Oshrain;A. Salkind;K. Siegel
对松散粘附性斑块(LAP)和粘附性斑块(AP)的病理潜能进行了一项研究，并用溶解分析法(LLA)对其进行了比较。设计了一种在无热原条件下从牙龈上和牙下收集AP和LAP的技术。受试者停止清洁牙齿两周后，从牙齿(G.I.=<；1)采集了5个牙龈上样本(AP和LAP)。放置1周后，在牙周袋深度不小于4 mm和不超过7 mm的牙周袋中采集5个牙龈下样本。所有的样本都被冷冻干燥，重建，并通过吲哚三酮反应定量分析斑块质量(Moore和Stein，1954)。通过对Levin和Bang(1964)的LLA进行修改，对样本进行了溶解物活性分析。五个牙龈上样本的结果表明，由金刚三酮反应确定的菌斑质量，AP组比LAP组大4-5倍。在五个牙龈下样本中，吲哚三酮在AP中的值是LAP的两倍。尽管斑块质量有这些差异，但当以每摩尔氨基酸的显色剂活性表示时，所有LAP样本中的显色反应活性始终高于可比AP样本。这些初步发现表明，位于战略重要位置的龈下LAP具有生物学活性，值得进一步研究。
A study was undertaken to assay and compare the pathological potential of loosely adherent plaque (LAP) with that of adherent plaque (AP) using the limulus lysate assay (LLA). A technique was devised for collection both AP And LAP from supra‐ as well as subgingival tooth areas under pyrogen‐free conditions. Five supragingival samples (AP & LAP) were collected from teeth (G.I.= < 1) after subjects had abstained from tooth cleaning for two weeks. Five subgingival samples were collected from mylar strips one week after placement in periodontal pockets not less than 4 mm and not more than 7 mm in depth. All samples were lyophilized, recontituted and analyzed for plaque mass by the quantitative ninhydrin reaction (Moore and Stein, 1954). Samples were analyzed for limulus lysate activity by a modification of the LLA of Levin and Bang (1964).Results from the five supragingival samples indicate that plaque mass, as determined by the ninhydrin reaction, was 4–5 fold greater in AP than in LAP. In the five subgingival samples, ninhydrin values for AP were two times higher than LAP. In spite of these differences in plaque mass, limulus activity in all LAP samples was consistently higher than in comparable AP samples when expressed as limulus activity per mole of amino acid. These preliminary findings suggest that subgingival LAP, situated in a position of strategic importance, is biologically active and deserves further study.
影响因子：
2
作者：
LOE, HARALD;SILNESS, JOHN
通讯作者：
SILNESS, JOHN
影响因子：
2
作者：
SILNESS, J;LOE, H
通讯作者：
LOE, H