Remarkable plasticity of Na(+), K(+)-ATPase, Ca(2+)-ATPase and SERCA contributes to muscle disuse atrophy resistance in hibernating Daurian ground squirrels.
Remarkable plasticity of Na(+), K(+)-ATPase, Ca(2+)-ATPase and SERCA contributes to muscle disuse atrophy resistance in hibernating Daurian ground squirrels.
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DOI：
10.1038/s41598-017-10829-6
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发表时间：
2017-09-05
影响因子：
4.6
通讯作者：
Gao Y
中科院分区：
文献类型：
--
作者：
Guo Q;Mi X;Sun X;Li X;Fu W;Xu S;Wang Q;Arfat Y;Wang H;Chang H;Gao Y
我们研究了冬眠的达乌尔黄鼠（Spermophilus dauricus），非冬眠的后肢卸载（HLU）松鼠和HLU大鼠骨骼肌纤维中的胞浆钙（Ca 2+）和肌浆网Ca 2+调节，以阐明参与防止冬眠动物肌肉萎缩的分子机制。结果表明，松鼠比目鱼肌Na+，K+-ATP酶和Ca 2 +-ATP酶活性在冬眠期保持不变，在间歇唤醒期（IB-A）下降，间歇唤醒后（Post-IBA）恢复到冬眠前（AUT/Pre-H）水平;趾长伸肌（EDL）活性在冬眠期保持不变，在冬眠后（Post-H）上升。活动增加的SOL的HLU大鼠，但在HLU松鼠稳定。肌浆网/内质网Ca ~（2+）-ATP酶（SERCA）活性在IB-A松鼠SOL中降低，但在Post-IBA组中恢复到AUT/Pre-H水平;在EDL中没有发现显著变化。SERCA活性在HLU松鼠的EDL和HLU大鼠的SOL中增加。与AUT/Pre-H相比，SERCA 2型蛋白在IB-A和Post-IBA松鼠的SOL和EDL中表达增加，但仅在HLU动物的SOL中表达增加。本文还描述了蛋白激酶A的变化。因此，冬眠地松鼠表现出显着的Na+，K+-ATP酶，Ca 2 +-ATP酶，和SERCA可塑性。
We investigated cytosolic calcium (Ca2+) and sarcoplasmic reticulum Ca2+ regulation in skeletal muscle fibers of hibernating Daurian ground squirrels (Spermophilus dauricus), non-hibernating hindlimb-unloaded (HLU) squirrels, and HLU rats to clarify the molecular mechanisms involved in preventing muscle atrophy in hibernators. The Na+, K+-ATPase and Ca2+-ATPase activities in the soleus muscle (SOL) of squirrels were maintained in hibernation, decreased during interbout arousal (IB-A), and increased to autumn/pre-hibernation (AUT/Pre-H) levels in torpor after interbout arousal (Post-IBA), whereas activities in the extensor digitorum longus muscle (EDL) were stable during hibernation, but increased during post-hibernation (Post-H). Activities increased in the SOL of HLU rats, but were stable in HLU squirrels. Sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) activity in the SOL decreased in IB-A squirrels, but returned to AUT/Pre-H levels in the Post-IBA group; no significant changes were found in the EDL. SERCA activity increased in the EDL of HLU squirrels and SOL of HLU rats. Compared with AUT/Pre-H, SERCA type 2 protein expression increased in the SOL and EDL of IB-A and Post-IBA squirrels, but increased in the SOL only in HLU animals. We also describe the protein kinase A changes in this paper. Thus, hibernating ground squirrels displayed remarkable Na+, K+-ATPase, Ca2+-ATPase, and SERCA plasticity.
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DOI：
10.1016/j.biocel.2013.06.011
发表时间：
2013-10
影响因子：
4
作者：
Bodine, Sue C.
通讯作者：
Bodine, Sue C.
显着保持 Ca2 稳态和抑制细胞凋亡有助于冬眠达乌尔地松鼠的抗肌肉萎缩作用
影响因子：
4.6
作者：
Fu W;Hu H;Dang K;Chang H;Du B;Wu X;Gao Y
通讯作者：
Gao Y
影响因子：
2.1
作者：
Stammers, Andrew N.;Susser, Shanel E.;Duhamel, Todd A.
通讯作者：
Duhamel, Todd A.
影响因子：
5.6
作者：
Lee, Kisoo;So, Hyekyoung;Choi, Inho
通讯作者：
Choi, Inho
稳定的 atrogin-1 (Fbxo32) 和 MuRF1 (Trim63) 基因表达参与冬眠达乌尔地松鼠 (Spermophilus dauricus) 比目鱼肌的保护机制
影响因子：
2.4
作者：
Dang K;Li YZ;Gong LC;Xue W;Wang HP;Goswami N;Gao YF
通讯作者：
Gao YF